A heterotrophic mutant of Chlorella sorokiniana MB-1-M12 was evaluated for its ability to produce lutein using organic carbon and nitrogen sources and without light irradiation. In batch fermentation, the maximal lutein content (3.67 mg lutein/g biomass) and productivity (2.84 mg/L/d) could be obtained when cultivated in BG-11 medium with 7.5 g/L glucose, 0.75 g/L urea, pH 7.5 and a C/N ratio of 10. A novel two-stage cultivation strategy that integrates fed-batch and semi-batch operations was applied to enhance the lutein production performance. When growing MB-1-M12 strain in a 5L fermenter using the optimal operation strategies, the maximum biomass concentration, biomass productivity, lutein content and lutein productivity could reach 25 g/L, 4.88 mg/L/d, 5.88 mg/g and 16.2 mg/L/d, respectively. This high lutein productivity could significantly reduce the cultivation time and the associated costs, indicating the potential of using MB-1-M12 strain for heterotrophic lutein production in commercial scale.

Sorokiniana小球藻的异养突变体评估了MB-1-M12在使用有机碳和氮源且无光照射的情况下生产叶黄素的能力。在分批发酵中，当在含有7.5 g / L葡萄糖，0.75 g / L尿素，pH值的BG-11培养基中培养时，可以获得最大的叶黄素含量（3.67 mg叶黄素/ g生物量）和生产率（2.84 mg / L / d）。 C / N比为7.5，C / N为10。将分批补料和半分批操作相结合的新型两阶段培养策略可提高叶黄素的生产性能。使用最佳操作策略在5L发酵罐中培养MB-1-M12菌株时，最大生物量浓度，生物量生产率，叶黄素含量和叶黄素生产率可以分别达到25 g / L，4.88 mg / L / d，5.88 mg / g和分别为16.2 mg / L / d。