A heterotrophic mutant of MB-1-M12 was evaluated for its ability to produce lutein using organic carbon and nitrogen sources and without light irradiation. In batch fermentation, the maximal lutein content (3.67 mg lutein/g biomass) and productivity (2.84 mg/L/d) could be obtained when cultivated in BG-11 medium with 7.5 g/L glucose, 0.75 g/L urea, pH 7.5 and a C/N ratio of 10. A novel two-stage cultivation strategy that integrates fed-batch and semi-batch operations was applied to enhance the lutein production performance. When growing MB-1-M12 strain in a 5L fermenter using the optimal operation strategies, the maximum biomass concentration, biomass productivity, lutein content and lutein productivity could reach 25 g/L, 4.88 mg/L/d, 5.88 mg/g and 16.2 mg/L/d, respectively. This high lutein productivity could significantly reduce the cultivation time and the associated costs, indicating the potential of using MB-1-M12 strain for heterotrophic lutein production in commercial scale.

中文翻译：

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利用异养培养小球藻 MB-1-M12 生产叶黄素的高效两阶段培养策略


评估了 MB-1-M12 的异养突变体使用有机碳和氮源且无需光照射生产叶黄素的能力。在分批发酵中，当在7.5 g/L葡萄糖、0.75 g/L尿素、pH值的BG-11培养基中培养时，可以获得最大叶黄素含量（3.67 mg叶黄素/g生物量）和生产率（2.84 mg/L/d）。 7.5，C/N 比为 10。采用集补料分批和半分批操作于一体的新型两阶段培养策略来提高叶黄素生产性能。采用最佳操作策略在5L发酵罐中培养MB-1-M12菌株时，最大生物量浓度、生物量生产率、叶黄素含量和叶黄素生产率分别达到25 g/L、4.88 mg/L/d、5.88 mg/g和分别为 16.2 毫克/升/天。这种高叶黄素生产率可以显着减少培养时间和相关成本，表明使用 MB-1-M12 菌株进行商业规模异养叶黄素生产的潜力。