Anaerobic co-digestion of various sewage sludges is a promising approach for greater recovery of energy, but the process is more complicated than mono-digestion of sewage sludge. The applicability of microbial structure analyses and gene quantification to understand microbial conditions was evaluated. The results show that information from gene analyses is useful in managing anaerobic co-digestion and damaged microbes in addition to conventional parameters like total solids, pH and biogas production. Total bacterial 16S rRNA gene copy numbers are the most useful tools for evaluating unstable anaerobic digestion of sewage sludge, rather than mcrA and total archaeal 16S rRNA gene copy numbers, and high-throughput sequencing. First order decay rates of gene copy numbers during pH failure were higher than typical decay rates of microbes in stable operation. The sequencing analyses, including multidimensional scaling, showed very different microbial structure shifts, but the results were not consistent.

中文翻译：

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各类污泥厌氧消化过程中微生物结构及基因拷贝数对比分析


各种污水污泥的厌氧共消化是一种有前途的更大程度地回收能量的方法，但该过程比污水污泥的单一消化更复杂。评估了微生物结构分析和基因定量在了解微生物条件方面的适用性。结果表明，除了总固体量、pH 值和沼气产量等常规参数外，基因分析信息还可用于管理厌氧共消化和受损微生物。细菌总 16S rRNA 基因拷贝数是评估污水污泥不稳定厌氧消化最有用的工具，而不是 mcrA 和总古菌 ​​16S rRNA 基因拷贝数以及高通量测序。 pH 失效期间基因拷贝数的一级衰减率高于稳定运行时微生物的典型衰减率。包括多维尺度在内的测序分析显示出非常不同的微生物结构变化，但结果并不一致。