Targeted and untargeted analyses of the sub-5 kDa urine metabolome of genitourinary cancer patients (prostate and/or bladder) were performed without chemical derivatization using capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS). For targeted analysis, endogenous levels of sarcosine and 5 other amino acid metabolites implicated in the progression of prostate cancer were quantified in four patients and in a pooled urine sample from healthy volunteers. An untargeted analysis (m/z 50 to 850) of patient urine was performed using the same CE-ESI-MS system identifying over 400 distinct molecular features per patient. All patient urine samples were collected at prostatectomy/cystectomy via catheter. Patient urine samples were filtered by centrifugation, with endogenous sarcosine enriched by solid-phase extraction, and the processed samples loaded onto CE-ESI-MS for analysis. Diagnostic information, digital pathological slides, and tissue samples were collected and stored in a comprehensive biobanking database. The introduction of urine sample collection into the surgery workflow was facile and is a promising strategy for addressing the translational research challenge of moving smoothly from “chromatogram to nomogram”.

使用毛细管电泳-电喷雾电离质谱（CE-ESI-MS），对泌尿生殖系统癌症患者（前列腺癌和/或膀胱癌）的5 kDa以下尿代谢组进行了有针对性和无针对性的分析。为了进行有针对性的分析，对四名患者和健康志愿者收集的尿液样本中的内源性肌氨酸和其他5种氨基酸代谢产物的水平与前列腺癌的发展进行了定量。非目标分析（m / z使用相同的CE-ESI-MS系统对50至850位患者的尿液进行了分析，确定了每个患者400多种不同的分子特征。通过前列腺切除术/膀胱切除术收集所有患者尿液样本。通过离心过滤患者尿液样品，并通过固相萃取富集内源性肌氨酸，并将处理后的样品上样至CE-ESI-MS上进行分析。收集诊断信息，数字病理切片和组织样本并将其存储在综合的生物库数据库中。将尿液样品收集引入手术流程很容易，并且是解决从“色谱图到列线图”平稳过渡的转化研究挑战的有前途的策略。