β-CD–GO composite film was immobilized on a glassy carbon electrode (GCE) to develop an electrochemical sensor for simultaneous determination of the isomers of Imperatorin (IMP) and Isoimperatorin (IIMP) and Umbelliferone (UB) for the first time. The electrochemical behaviors of the three components were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The peak-to-peak potential differences between IMP and IIMP, IMP and UB, and IIMP and UB were 0.135, 0.540 and 0.405 V, respectively. The calibration curves for IMP, IIMP and UB were obtained in the range of 2.5–90.0 μM, 3.0–90.0 μM and 0.8–20.0 μM with the limits of detection of 0.5, 0.8 and 0.05 μM (S/N = 3), respectively. The modified electrode was successfully applied for the simultaneous determination of IMP, IIMP and UB in traditional Chinese medicine Angelica dahuricae Radix and Duliang Diwan with excellent recoveries as confirmed by HPLC method.

将β-CD-GO复合膜固定在玻璃碳电极（GCE）上，以开发出一种电化学传感器，用于同时测定欧前胡素（IMP）和异欧前胡素（IIMP）和伞形酮（UB）的异构体。通过循环伏安法（CV），电化学阻抗谱法（EIS）和差动脉冲伏安法（DPV）研究了这三种组分的电化学行为。IMP和IIMP，IMP和UB以及IIMP和UB之间的峰峰电位差分别为0.135 V，0.540 V和0.405V。IMP，IIMP和UB的校准曲线分别在2.5–90.0μM，3.0–90.0μM和0.8–20.0μM的范围内获得，检出限分别为0.5、0.8和0.05μM（S / N = 3）。 。修饰电极已成功应用于同时测定IMP，HPLC法证实白当归和独良地丸具有极好的回收率。