Background and aims

Development of high-performance submucosal injection materials (SIMs) contribute to the advancement of endoscopic therapy for early-stage gastrointestinal neoplasms. This study aimed to develop a new ex vivo model that mimics the human gastrointestinal tract to evaluate the performance (the height and duration of the submucosal elevation) of various SIMs in detail.

Methods

A new ex vivo model that applies a constant tension to the tested specimen (the porcine gastric specimen) was developed. SIMs were injected into the submucosa at the center or edge of the tested specimen, and submucosal elevation heights (SEHs) were measured over time.

Results

The average value and standard deviation of SEH determined using the conventional model (the tested specimen was fixed with pins) were higher than those obtained using the new model, which showed that the new model could precisely measure the SEH of a given SIM. In addition, the performance (SEH) of SIMs decreased with increasing tension applied to the specimen, suggesting that the performance of SIMs deteriorates with the over-expansion of the gastrointestinal tract. The submucosal elevation formed at the specimen edge disappeared faster than that formed at the specimen's center.

Conclusions

The proposed new ex vivo model allows accurate SEH measurement under uniform conditions and detailed comparison of the performances of various types of SIMs and can contribute to the development of high-performance materials.

中文翻译：

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开发用于评估内窥镜下粘膜下注射材料性能的新型离体模型

背景和目标

高性能粘膜下注射材料（SIMs）的开发有助于早期胃肠道肿瘤的内镜治疗的发展。这项研究旨在开发一种新的体外模型，该模型模仿人的胃肠道，以详细评估各种SIM卡的性能（粘膜下抬高的高度和持续时间）。

方法

开发了一种新的离体模型，该模型将恒定的张力施加到测试样本（猪胃样本）上。将SIM注射到被测样品中心或边缘的粘膜下层，并随时间测量粘膜下高度（SEH）。

结果

使用常规模型（测试样品用销钉固定）确定的SEH平均值和标准偏差高于使用新模型获得的SEH平均值和标准偏差，这表明新模型可以精确地测量给定SIM卡的SEH。此外，SIMs的性能（SEH）随着施加到样品上的张力的增加而降低，这表明SIMs的性能会随着胃肠道的过度扩张而恶化。在标本边缘形成的粘膜下抬高消失的速度比在标本中心形成的粘膜下抬高的消失快。

结论

提出的新型离体模型可以在统一条件下进行准确的SEH测量，并可以对各种类型的SIM卡的性能进行详细比较，并有助于开发高性能材料。