The aim of this study was to examine the effect of process factors such as ethanol concentration, extraction time and temperature on the extraction yield and the bioactive contents of Tagetes lemmonii leaf extracts using response surface methodology (RSM). ANOVA results showed that the response variables were affected by the ethanol concentration to a very significant degree and by extraction temperature to a lesser degree. GC/MS characterization showed that the extract is rich in bioactive compounds and those present exhibited important biological activities such as antioxidant, insect repellence and insecticidal activities. The results from the toxicity assay demonstrate that the extract obtained from the leaves of Tagetes lemmonii was an effective insect toxin against Tribolium castaneum. The radical scavenging activity and p-anisidine test results of olive oil spiked with different concentrations of leaf extract showed that the phenolic compounds can retard lipid oxidation.

这项研究的目的是使用响应表面方法（RSM）研究乙醇浓度，提取时间和温度等工艺因素对柠檬蒿万寿菊叶提取物的提取率和生物活性含量的影响。方差分析结果表明，响应变量受乙醇浓度的影响很大，而提取温度的影响较小。GC / MS表征表明，提取物富含生物活性化合物，并且所存在的化合物表现出重要的生物活性，例如抗氧化剂，驱虫性和杀虫活性。毒性试验的结果表明，从万寿菊的叶子中提取的提取物是一种有效的抗昆虫毒素。bol藜属（Tribolium castaneum）。不同浓度的叶提取物加标橄榄油的自由基清除活性和对氨基苯甲酸测试结果表明，酚类化合物可以抑制脂质氧化。