Abstract Botryococcus braunii is a colonial microalga that produces hydrocarbons. While the microalga stores almost all the amount of hydrocarbons in its colony matrix, the amount of the extracted hydrocarbon without any pretreatment is typically very small. We performed mechanical cell disruption using a JET PASTER® and a bead mill as the pretreatment ways to facilitate hydrocarbon extraction from B. braunii . After the disruption, the size and shape of colonies changed. In the JET PASTER treatment at 4800 rpm, the concentration of removed polysaccharides increased 146 to 173 μg/mL and the hydrocarbon yield increased 2.7 to 82.8%. In the bead mill treatment, the concentration of removed polysaccharides increased 146 to 210 μg/mL and hydrocarbon yield increased 2.7 to 42.3%. Therefore, the disruption of colonies and polysaccharides around algal colonies would affect the hydrocarbon extraction. In addition, the apparent photosynthetic activity of the sample treated by the JET PASTER was 0.71 that is almost the same value as that of the untreated sample, whereas that of the sample treated by the bead mill was 0.64. Therefore, the JET PASTER treatment did not affect the photosynthetic function of B. braunii .

中文翻译：

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布氏葡萄球菌烃提取过程中菌落破坏的研究

摘要 Botryococcus braunii 是一种能产生碳氢化合物的群落微藻。虽然微藻在其菌落基质中储存了几乎所有数量的碳氢化合物，但未经任何预处理的提取碳氢化合物的量通常非常少。我们使用 JET PASTER® 和珠磨机进行机械细胞破碎，作为促进从 B. braunii 中提取碳氢化合物的预处理方法。破坏后，菌落的大小和形状发生了变化。在 4800 rpm 的 JET PASTER 处理中，去除的多糖浓度增加了 146 至 173 μg/mL，烃产率增加了 2.7% 至 82.8%。在珠磨处理中，去除的多糖浓度增加了 146 至 210 μg/mL，烃产率增加了 2.7% 至 42.3%。所以，藻类菌落周围菌落和多糖的破坏会影响碳氢化合物的提取。此外，经 JET PASTER 处理的样品的表观光合活性为 0.71，与未处理样品的值几乎相同，而经珠磨机处理的样品的表观光合活性为 0.64。因此，JET PASTER 处理不影响 B. braunii 的光合功能。