In this study, the aggregation of nine pyridine-degrading strains was investigated for the acceleration of the aerobic granulation process, with emphasis on the coaggregation mechanisms involved. Rhizobium sp. NJUST18 and Shinella granuli NJUST29 (with strong aggregation ability but with relatively poor pyridine degradation ability) and Paracoccus versutus NJUST32 (with strong pyridine degradation ability but with poor aggregation ability) were chosen as inocula for aerobic granulation. The combination of NJUST18 + NJUST29 demonstrated more powerful coaggregation abilities and comparable pyridine-degrading ability compared with NJUST18 + NJUST32, NJUST29 + NJUST32 and the individual strains. The investigation of the coaggregation mechanism indicated that the combination of NJUST18 + NJUST29 presented the highest extracellular polymeric substances (EPS) content of 232.79 mg gVSS⁻¹ and the second messenger cyclic diguanylate (c-di-GMP) content of 268.56 mg gVSS⁻¹, making the combination beneficial for aerobic granulation. In the sequential batch reactors (SBRs) inoculated with the combination of NJUST18 + NJUST29, aerobic granules with diameter of 0.2–0.5 mm was formed after operation period as short as 42 days, demonstrating that the combination of NJUST18 + NJUST29 as inocula in aerobic granulation system had promising potential for the treatment of high-strength pyridine wastewater.

在这项研究中，研究了九种降解吡啶的菌株的聚集，以促进需氧制粒过程的加速，并着重于所涉及的共聚集机制。根瘤菌属。NJUST18和Shinella granuli NJUST29（具有很强的聚集能力，但吡啶降解能力相对较差）和副球菌选择NJUST32（吡啶降解能力强但聚集能力差）作为好氧制粒接种物。与NJUST18 + NJUST32，NJUST29 + NJUST32和个别菌株相比，NJUST18 + NJUST29的组合显示出更强大的共聚能力和相当的吡啶降解能力。对共聚集机制的研究表明，NJUST18 + NJUST29的组合显示出最高的细胞外聚合物（EPS）含量为232.79 mg gVSS ^-1和第二种信使环状双鸟苷酸酯（c-di-GMP）含量为268.56 mg gVSS ^-1，使该组合有益于有氧造粒。在使用NJUST18 + NJUST29组合接种的连续分批反应器（SBR）中，在短短42天的运行时间后，形成了直径为0.2-0.5 mm的好氧颗粒，表明NJUST18 + NJUST29的组合作为有氧造粒的接种物该系统在处理高强度吡啶废水中具有广阔的应用前景。