Introduced in 2000, atmospheric pressure (AP)/matrix-assisted laser desorption/ionization (MALDI) has attracted substantial attention in the mass spectrometry community due to its ease of sample introduction and handling, interchangeability with ESI source and capability of analyzing volatile species. In this study, an AP/MALDI source with ultra-high spatial resolution was coupled to a Q Exactive HF orbitrap mass spectrometer for high resolution in situ analysis by MALDI, laserspray ionization (LSI) and matrix assisted ionization (MAI) without instrument modification. LSI and MAI generated multiply charged ions, which expanded the mass detection range and improved fragmentation efficiency. Full MS, targeted MS/MS, data dependent acquisition (DDA) and parallel reaction monitoring (PRM) acquisitions were performed on peptide and protein standards, tissue extracts and tissue sections for in depth characterization of various biomolecules. High resolution full MS and MS/MS images were obtained from crustacean and rat tissues with pixel size less than 30 μm. Overall, AP/MALDI-Q-Orbitrap is a fast scanning instrument that is capable of performing multiple types of ionization and multiple acquisition modes without instrument modification. This instrument platform provides an attractive alternative to other high resolution MALDI instruments.

中文翻译：

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高分辨率大气压基质辅助激光解吸/电离-四极杆-轨道阱 MS 平台可通过多模式电离和采集对生物分子进行原位分析

大气压 (AP)/基质辅助激光解吸/电离 (MALDI) 于 2000 年推出，由于其易于样品引入和处理、与 ESI 源的互换性以及分析挥发性物质的能力，在质谱界引起了广泛关注。在这项研究中，具有超高空间分辨率的 AP/MALDI 源与 Q Exactive HF 轨道阱质谱仪耦合，用于通过 MALDI、激光喷雾电离 (LSI) 和基质辅助电离 (MAI) 进行高分辨率原位分析，无需仪器修改。LSI 和 MAI 生成多电荷离子，从而扩大了质量检测范围并提高了碎裂效率。对肽和蛋白质标准品进行全质谱、靶向 MS/MS、数据相关采集 (DDA) 和平行反应监测 (PRM) 采集，用于深入表征各种生物分子的组织提取物和组织切片。从像素尺寸小于 30 μm 的甲壳类动物和大鼠组织中获得高分辨率全 MS 和 MS/MS 图像。总体而言，AP/MALDI-Q-Orbitrap 是一种快速扫描仪器，无需修改仪器即可执行多种类型的电离和多种采集模式。该仪器平台为其他高分辨率 MALDI 仪器提供了一个有吸引力的替代方案。AP/MALDI-Q-Orbitrap 是一种快速扫描仪器，无需修改仪器即可执行多种类型的电离和多种采集模式。该仪器平台为其他高分辨率 MALDI 仪器提供了一个有吸引力的替代方案。AP/MALDI-Q-Orbitrap 是一种快速扫描仪器，无需修改仪器即可执行多种类型的电离和多种采集模式。该仪器平台为其他高分辨率 MALDI 仪器提供了一个有吸引力的替代方案。