Methoxyindoles have been of continued interest due to their biological effects. Recent studies using enzyme-linked immunosorbent assays (ELISAs) have reported micromolar levels of 5-methoxytryptophan (MeOTRP) in human plasma. This prompted our development of a method for the determination of MeOTRP in human plasma that involved solid phase extraction (SPE) followed by HPLC. The identity of a putative MeOTRP peak was confirmed through chromatographic, voltammometric and fluorometric characterization. Using a standard addition approach, actual mean human plasma MeOTRP levels were found to be approximately 2 ng/mL, 20 to 100 times lower than previously reported. Prior studies on the physiological and possible biomarker roles of MeOTRP need to be reconsidered and future studies of MeOTRP need to take the present findings into consideration.

甲氧基吲哚由于其生物学作用而引起了人们的持续关注。使用酶联免疫吸附测定（ELISA）的最新研究已经报道了人血浆中5甲氧基色氨酸（MeOTRP）的微摩尔水平。这促使我们开发了一种测定人血浆中MeOTRP的方法，该方法涉及固相萃取（SPE），然后进行HPLC。通过色谱，伏安法和荧光法表征确认推定的MeOTRP峰的身份。使用标准添加方法，发现人血浆MeOTRP的实际平均水平约为2 ng / mL，比先前报道的水平低20至100倍。需要重新考虑有关MeOTRP的生理和可能的生物标志物作用的先前研究，并且未来对MeOTRP的研究需要考虑到目前的发现。