The physicochemical characteristics and in vitro antioxidant and in vivo anti-ageing activities of partial purified Chlorella pyrenoidosa polysaccharides (PCPPs) were investigated. The building blocks of PCPPs were mainly composed of D-glucose, D-galactose and D-mannose. The average molecular weight of PCPPs was 9,950 Da. In vitro antioxidant activity assays showed that PCPPs could effectively scavenge hydroxyl, 1,1-diphenyl-2-picrylhydrazyl, and superoxide radicals, with stronger effect on hydroxyl radicals. Furthermore, the mean lifespan of the male and female Drosophila melanogaster was extended by 11.5% and 10.6%, respectively. This was accompanied by an increase in the total activity of the endogenous antioxidant enzymes, superoxide dismutase, glutathione peroxidase, and catalase in young or old D. melanogaster administered with PCPPs. Moreover, a gender-dependent difference was observed both in lifespan and antioxidant enzyme activities in D. melanogaster. The results indicated that C. pyrenoidosa polysaccharides are potential natural antioxidants in extending lifespan.

研究了部分纯化的小球藻小球藻多糖（PCPPs）的理化特性，体外抗氧化剂和体内抗衰老活性。PCPPs的组成部分主要由D-葡萄糖，D-半乳糖和D-甘露糖组成。PCPP的平均分子量为9,950 Da。体外抗氧化活性分析表明，五氯苯酚可有效清除羟基，1，1-二苯基-2-吡啶并肼基和超氧自由基，对羟自由基的作用更强。此外，男性和女性果蝇的平均寿命分别扩大了11.5％和10.6％。这伴随着给予PCPP的年轻或老年黑腹果蝇的内源性抗氧化酶，超氧化物歧化酶，谷胱甘肽过氧化物酶和过氧化氢酶的总活性增加。此外，在D. melanogaster的寿命和抗氧化酶活性方面均观察到性别依赖性差异。结果表明，C。pyrenoidosa多糖是延长寿命的潜在天然抗氧化剂。