Generation of mucin gel particles with self-degradable and -releasable properties†,Journal of Materials Chemistry B

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Generation of mucin gel particles with self-degradable and -releasable properties†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2018-01-05 00:00:00 , DOI: 10.1039/c7tb02663c
Yuuka Fukui _{1,

2,

3,

4,

5} , Megumi Fukuda _{1,

2,

3,

4,

5} , Keiji Fujimoto _{1,

2,

3,

4,

5}

Affiliation

Herein, we focused on mucin, which is a large viscous glycoprotein in terms of materials science, and reported preparation of mucin gel particles and incorporation of enzymes to provide the particle with self-degradable and releasable properties. To expose the hydrophobic peptide cores, trimming of sugar moieties was carried out by β-elimination reaction under alkaline conditions (tMucin). Nano-sized tMucin particles were prepared by the assembly of tMucin with the aid of a cationic surfactant. Then, cross-linking of tMucin particles was carried out via heat treatment (annealing) to induce thermal aggregation of the polypeptide chains. The hydrodynamic diameter of tMucin particles reversibly changed in response to calcium ions. Next, in an attempt to render the particle degradable, lysozyme was incorporated into the tMucin particles for the hydrolysis of oligosaccharide chains. These particles were gradually degraded upon enzymatic cleavage of the mucin molecules, facilitating the release of their incorporated substances. Also, the degradation of the mucin particles and the release of lysozyme were tunable by environmental conditions, such as temperature and calcium ions, in addition to the degree of cross-linking of the particles.

中文翻译：

产生具有自降解和可释放特性的粘蛋白凝胶颗粒†

在本文中，我们着眼于粘蛋白，它在材料科学方面是一种大的粘性糖蛋白，并报道了粘蛋白凝胶颗粒的制备和酶的掺入以提供具有自降解和可释放特性的颗粒。为了暴露疏水性肽核心，在碱性条件下（tMucin）通过β-消除反应进行糖部分的修整。通过在阳离子表面活性剂的帮助下组装tMucin制备纳米级tMucin颗粒。然后，通过热处理（退火）以诱导多肽链的热聚集。响应钙离子，tMucin颗粒的流体动力学直径可逆地变化。接下来，为了使颗粒可降解，将溶菌酶掺入tMucin颗粒中以水解寡糖链。这些颗粒在粘蛋白分子的酶促裂解后逐渐降解，从而促进其结合物质的释放。而且，除了颗粒的交联度之外，粘蛋白颗粒的降解和溶菌酶的释放还可以通过环境条件调节，例如温度和钙离子。

更新日期：2018-01-05

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