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Tailoring the Efficacy of Multifunctional Biopolymeric Graphene Oxide Quantum Dot-Based Nanomaterial as Nanocargo in Cancer Therapeutic Application
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2018-01-30 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00689 Sriparna De 1 , Kartick Patra 2 , Debatri Ghosh 3 , Koushik Dutta 1 , Aditi Dey 4 , Gunjan Sarkar 1 , Jyotirmay Maiti 2 , Arijita Basu 1 , Dipak Rana 5 , Dipankar Chattopadhyay 1
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2018-01-30 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00689 Sriparna De 1 , Kartick Patra 2 , Debatri Ghosh 3 , Koushik Dutta 1 , Aditi Dey 4 , Gunjan Sarkar 1 , Jyotirmay Maiti 2 , Arijita Basu 1 , Dipak Rana 5 , Dipankar Chattopadhyay 1
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Nanotechnology has acquired an immense recognition in cancer theranostics. Considerable progress has been made in the development of targeted drug delivery system for potent delivery of anticancer drugs to tumor-specific sites. Recently, multifunctional nanomaterials have been explored and used as nanovehicles to carry drug molecules with enhanced therapeutic efficacy. In this present work, graphene oxide quantum dot (GOQD) was conjugated with folic acid functionalized chitosan (FA-CH) to develop a nanocargo (FA-CH-GOQD) for drug delivery in cancer therapy. The synthesized nanomaterials were characterized using Fourier transform infrared spectroscopy, ultraviolet–visible spectroscopy, scanning electron microscopy, transmission electron microscopy, and dynamic light scattering. Photoluminescence spectroscopy was also employed to characterize the formation of GOQD. To validate the efficacy of FA-CH-GOQD as nanocarriers, doxorubicin (DOX) drug was chosen for encapsulation. The in vitro release pattern of DOX was examined in various pH ranges. The drug release rate in a tumor cell microenvironment at pH 5.5 was found higher than that under a physiological range of pH 6.5 and 7.4. An MTT assay was performed to understand the cytotoxic behavior of GOQD and FA-CH-GOQD/DOX. Cytomorphological micrographs of the A549 cell exhibited the various morphological arrangements subject to apoptosis of the cell. Cellular uptake studies manifested that FA-CH-GOQD could specifically transport DOX within a cancerous cell. Further anticancer efficacy of this nanomaterial was corroborated in a breast cancer cell line and demonstrated through 4′,6-diamidino-2-phenylindole dihydrochloride staining micrographs.
中文翻译：
调整多功能生物聚合氧化石墨烯量子点基纳米材料作为纳米货物在癌症治疗中的功效
纳米技术已在癌症治疗学中获得了巨大的认可。在靶向药物递送系统的开发中已经取得了相当大的进展，该靶向药物递送系统用于将抗癌药物有效地递送至肿瘤特异性部位。近来，已经研究了多功能纳米材料并将其用作载有具有增强治疗功效的药物分子的纳米载体。在这项目前的工作中，将氧化石墨烯量子点（GOQD）与叶酸官能化壳聚糖（FA-CH）共轭，以开发用于癌症治疗中药物递送的纳米货物（FA-CH-GOQD）。使用傅立叶变换红外光谱，紫外可见光谱，扫描电子显微镜，透射电子显微镜和动态光散射对合成的纳米材料进行了表征。还使用光致发光光谱法表征GOQD的形成。为了验证FA-CH-GOQD作为纳米载体的功效，选择了阿霉素（DOX）药物进行封装。在各种pH范围内检查了DOX的体外释放模式。发现在pH 5.5的肿瘤细胞微环境中的药物释放速率高于在pH 6.5和7.4的生理范围内的药物释放速率。进行MTT测定以了解GOQD和FA-CH-GOQD / DOX的细胞毒性行为。A549细胞的细胞形态显微照片显示出受细胞凋亡影响的各种形态排列。细胞摄取研究表明，FA-CH-GOQD可以在癌细胞内特异性转运DOX。
更新日期：2018-01-30
中文翻译：
调整多功能生物聚合氧化石墨烯量子点基纳米材料作为纳米货物在癌症治疗中的功效
纳米技术已在癌症治疗学中获得了巨大的认可。在靶向药物递送系统的开发中已经取得了相当大的进展，该靶向药物递送系统用于将抗癌药物有效地递送至肿瘤特异性部位。近来，已经研究了多功能纳米材料并将其用作载有具有增强治疗功效的药物分子的纳米载体。在这项目前的工作中，将氧化石墨烯量子点（GOQD）与叶酸官能化壳聚糖（FA-CH）共轭，以开发用于癌症治疗中药物递送的纳米货物（FA-CH-GOQD）。使用傅立叶变换红外光谱，紫外可见光谱，扫描电子显微镜，透射电子显微镜和动态光散射对合成的纳米材料进行了表征。还使用光致发光光谱法表征GOQD的形成。为了验证FA-CH-GOQD作为纳米载体的功效，选择了阿霉素（DOX）药物进行封装。在各种pH范围内检查了DOX的体外释放模式。发现在pH 5.5的肿瘤细胞微环境中的药物释放速率高于在pH 6.5和7.4的生理范围内的药物释放速率。进行MTT测定以了解GOQD和FA-CH-GOQD / DOX的细胞毒性行为。A549细胞的细胞形态显微照片显示出受细胞凋亡影响的各种形态排列。细胞摄取研究表明，FA-CH-GOQD可以在癌细胞内特异性转运DOX。
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