In the present study, potential protective role of Vitamin C (l-ascorbic acid) was investigated in aquaria acclimated common carp (Cyprinus carpio) following exposure for 96 h to combined toxic doses of fipronil (FP) and buprofezin (BPFN) insecticides in combination (FP: 200 μg/L; 4.57 × 10⁻⁷ mol/L and BPFN: 50 mg/L; 1.64 × 10⁻⁴ mol/L). At end of 96 h exposure, fish were supplemented with low (25 mg/L) and high (50 mg/L) doses of Vitamin C, added once daily to aquaria water for continuous three weeks. Appropriate control groups were run in parallel. Fish behavior was monitored throughout for signs of toxicity. At completion of experiments, liver, kidney, brain and gills were excised for toxicity assessment and possible remediation by the Vitamin C through biochemical determination of reactive oxygen species (ROS), thiobarbituric acid reactive substances or TBARS, reduced glutathione (GSH) and total protein content, levels of catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD), and the Comet assay. Hepatosomatic index (HSI), condition factor (CF), survival rate (SR), and combination index (CI) were also determined. Data were compared statistically at p < 0.05. Results showed significant behavioral and biochemical alterations, and DNA damage in the fish group exposed to FP and BPFN in combination. In fish groups supplemented with Vitamin C following FP and BPFN treatment, significant alleviation in tissue damage and toxic effects was represented by substantial decreases in ROS and TBARS production (p < 0.001), along with a concomitant significant increase in the survival rate, GSH and total protein content, HSI, CF, and activities of SOD, CAT and POD enzymes (p < 0.001). Mean tail length of comet and percent tail DNA decreased significantly (p < 0.001), which indicated amelioration of DNA damage. The study concludes that Vitamin C is an effective remedial treatment against FP and BPFN-induced damage in exposed fish.

在本研究中，研究了维生素C（1-抗坏血酸）在水族适应的鲤鱼（Cyprinus carpio）中暴露于组合毒性剂量的氟虫腈（FP）和丁丙非嗪（BPFN）杀虫剂组合96小时后的潜在保护作用。（FP：200μg/ L; 4.57×10 ^-7  mol / L和BPFN：50 mg / L; 1.64×10 ^-4 摩尔/升）。在暴露96小时后，向鱼补充低剂量（25 mg / L）和高剂量（50 mg / L）的维生素C，每天向水族馆水添加一次，连续三周。适当的对照组平行进行。整个过程中都要监测鱼的行为，以检查是否有毒性迹象。实验完成后，切除肝脏，肾脏，大脑和g片进行毒性评估，并通过生化测定活性氧（ROS），硫代巴比妥酸活性物质或TBARS，还原型谷胱甘肽（GSH）和总蛋白的维生素C进行补救含量，过氧化氢酶（CAT），超氧化物歧化酶（SOD）和过氧化物酶（POD）的水平，以及彗星分析。还确定了肝体指数（HSI），条件因子（CF），存活率（SR）和组合指数（CI）。在处对数据进行统计学比较p  ＜0.05。结果显示，在暴露于FP和BPFN的鱼类组中，行为和生化变化显着，DNA损伤。在FP和BPFN处理后补充维生素C的鱼群中，ROS和TBARS产量显着下降（p <  0.001），以及相应的成活率，GSH和GSH显着增加，代表着组织损伤和毒性的显着减轻。总蛋白质含量，HSI，CF以及SOD，CAT和POD酶的活性（p <  0.001）。平均彗星尾巴长度和尾巴DNA百分比显着降低（p < 0.001），表明DNA损伤有所改善。该研究得出的结论是，维生素C是一种有效的补救措施，可以应对暴露于鱼类中的FP和BPFN诱导的损害。