Dermal defects caused by trauma or disease are challenging to treat due to difficult-to-treat infections that impair wound healing. Due to the widespread emergence of drug-resistant bacteria and dwindling discoveries of new antibiotics, there is currently an urgent need to introduce novel antimicrobials effective against antibiotic-resistant bacteria without causing damage to host tissues. As selenium (Se) and silver (Ag) are known for their antimicrobial properties, we investigated the separate loading of these materials into porous chitosan/PVA (CS) scaffolds through a simple in situ deposition method to create two distinct wound dressing materials (CS-Se and CS-Ag). Scaffolds with Se nanostructures and scaffolds containing Ag nanostructures were characterized and their activities against S. aureus – (a Gram-positive bacterium), E. coli – (a Gram-negative bacterium) and Methicillin-Resistant S. aureus (MRSA) – (a multi-drug resistant bacterium) were compared. The release of Ag and Se in vitro was shown to depend strongly on the release medium used (deionised water, mammalian or bacterial culture media). Ag-loaded scaffolds showed a significant reduction in CFUs and cytotoxicity towards fibroblasts while Se-loaded scaffolds showed abilities to damage bacterial cell membrane and non-toxicity to fibroblast. Overall, in this study we have demonstrated simple, in situ immobilization porous CS scaffolds with either Se or Ag nanostructures which could be used to suit different wound healing applications.

由于难以治疗的感染会损害伤口愈合，因此由创伤或疾病引起的皮肤缺陷很难治疗。由于耐药细菌的广泛出现和新抗生素的发现日益减少，目前迫切需要引入对抗生素耐药细菌有效而又不损害宿主组织的新型抗菌剂。由于硒（Se）和银（Ag）以其抗菌特性而闻名，因此我们通过简单的原位沉积方法研究了将这些材料分别负载到多孔壳聚糖/ PVA（CS）支架中的过程，以创建两种不同的伤口敷料（CS） -Se和CS-Ag）。表征具有Se纳米结构的支架和含有Ag纳米结构的支架，以及它们对s的活性比较了金黄色葡萄球菌（一种革兰氏阳性细菌），大肠杆菌（一种革兰氏阴性细菌）和耐甲氧西林的金黄色葡萄球菌（MRSA）（一种耐多药细菌）。已证明在体外Ag和Se的释放在很大程度上取决于所用的释放介质（去离子水，哺乳动物或细菌培养基）。载有Ag的支架显示出CFU和对成纤维细胞的细胞毒性显着降低，而载有Se的支架显示出破坏细菌细胞膜的能力和对成纤维细胞的无毒性。总体而言，在这项研究中，我们证明了具有Se或Ag纳米结构的简单的原位固定多孔CS支架，可用于适应不同的伤口愈合应用。