Background

Diagnosis of fibrous tumors can be challenging and expensive due to the use of special stains.

Objective

Determine the usefulness of fluorescence microscopy in the evaluation of elastic tissue patterns on hematoxylin-eosin–stained slides.

Methods

In total, 228 slides representing different fibrous tumors were evaluated for their elastic tissue patterns by fluorescence microscopy, and sensitivity and specificity were determined for relevant comparisons.

Results

Fluorescence microscopy was found to be useful, especially for distinguishing dermatofibroma from dermatofibrosarcoma protuberans and dermatomyofibroma from other fibrous tumors.

Limitations

In some cases, excessive background staining made patterns difficult to interpret.

Conclusion

Evaluation of elastic tissue patterns by fluorescence microscopy in fibrous tumors is a cheap and efficient means to further delineate these often challenging tumors.

中文翻译：

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荧光显微镜用于评估苏木精-伊红染色载玻片上皮肤纤维增生内的弹性组织模式

背景

由于使用特殊染色剂，纤维肿瘤的诊断可能具有挑战性且成本高昂。

客观的

确定荧光显微镜在评估苏木精-伊红染色载玻片上的弹性组织模式中的有用性。

方法

总共通过荧光显微镜评估了 228 张代表不同纤维肿瘤的载玻片的弹性组织模式，并确定了相关比较的敏感性和特异性。

结果

发现荧光显微镜检查是有用的，特别是用于区分皮肤纤维瘤和皮肤纤维肉瘤突起以及皮肤肌纤维瘤和其他纤维性肿瘤。

限制

在某些情况下，过度的背景染色使模式难以解释。

结论

通过荧光显微镜评估纤维肿瘤中的弹性组织模式是一种廉价且有效的方法，可以进一步描绘这些通常具有挑战性的肿瘤。