Guanidinium salt solutions are one of the important electrolytes which have been extensively used for understanding the folding/unfolding mechanism of proteins. The structure of aqueous Gdm₂CO₃ has been controversial as some studies suggest that the hetero ion pairing takes place which leads to the aggregation whereas other studies does not support the idea of ion pairing. In this paper, we have used the steady state, time resolved fluorescence methods and density functional theory to understand the structure of guanidinium carbonate (Gdm₂CO₃) in terms of ion pairing as well as aggregation. The structure of the aqueous Gdm₂CO₃ has been controversial as some studies suggests that the Interestingly, Gdm₂CO₃ does not have any aromatic chromophore but it shows the broad visible fluorescence in the aqueous medium. Moreover, the fluorescence spectrum depicts a strong excitation dependent feature violating the Kasha's Rule. The concentration dependent emission, excitation, lifetime fluorescence along the density functional theory studies clearly suggests the formation of different size of aggregated clusters of Gdm₂CO₃ which causes the visible fluorescence of aqueous Gdm₂CO₃ violating the Kasha's rule.

胍盐溶液是重要的电解质之一，已广泛用于理解蛋白质的折叠/展开机制。Gdm ₂ CO ₃水溶液的结构一直是有争议的，因为一些研究表明发生杂离子配对会导致聚集，而其他研究则不支持离子配对的想法。在本文中，我们使用稳态，时间分辨荧光方法和密度泛函理论从离子对和聚集的角度了解了碳酸胍（Gdm ₂ CO ₃）的结构。Gdm ₂ CO ₃水溶液的结构由于一些研究表明，Gdm ₂ CO ₃没有任何芳香族发色团，但在水性介质中显示出宽的可见荧光，这一点一直是有争议的。此外，荧光光谱描述了强烈的依赖于激发的特征，违反了卡沙定律。沿密度泛函理论研究的浓度依赖性发射，激发，寿命荧光均清楚地表明，形成了不同大小的Gdm ₂ CO ₃聚集簇，这导致含水Gdm ₂ CO ₃的可见荧光违反了Kasha规则。