Two ultrasound-assisted pretreatment technologies, ultrasound-assisted alkaline and ultrasound-assisted aqueous ionic liquid tetrabutylammonium hydroxide ([TBA][OH]), are compared systematically in regard to enzymatic saccharification. Pretreated Eucalyptus samples were characterized by powder X-ray diffraction, ¹³C cross polarization/magic-angle spinning solid state NMR spectroscopy, Fourier transform infrared spectroscopy, Scanning electron microscope (SEM) and chemistry composition analysis. These results not only explain the enzymatic saccharification difference between samples from the microstructure level, but also provide helpful information for relevant pretreatment research. Ultrasound-assisted [TBA][OH] pretreatment acquired a significant enhancement in the initial enzymatic rate of cellulose (79.39 mg/g/h), and a reducing sugar yield of 426.6 mg/g at 48 h. The pretreatment combining inexpensive aqueous ionic liquid and ultrasound may provide a promising strategy in the field of bio-refinery because of its unique advantages.

系统地比较了两种超声辅助预处理技术，即超声辅助碱性和超声辅助水性离子液体氢氧化四丁基铵（[TBA] [OH]）的糖化糖化效果。预处理的桉树样品通过粉末X射线衍射进行表征，¹³C交叉极化/魔角旋转固态NMR光谱，傅立叶变换红外光谱，扫描电子显微镜（SEM）和化学成分分析。这些结果不仅从微观结构上解释了样品之间的酶促糖化差异，而且为相关的预处理研究提供了有益的信息。超声辅助的[TBA] [OH]预处理可显着提高纤维素的初始酶促速率（79.39 mg / g / h），并在48 h时降低糖产量426.6 mg / g。由于廉价的离子液体和超声波相结合的预处理，由于其独特的优势，可能在生物精炼领域提供有前途的策略。