Meat Science ( IF 5.7 ) Pub Date : 2018-01-05 , DOI: 10.1016/j.meatsci.2018.01.003 Jessica Moraes Malheiros , Cruz Elena Enríquez-Valencia , Bruno Oliveira da Silva Duran , Tassiana Gutierrez de Paula , Rogério Abdallah Curi , Josineudson Augusto I.I. de Vasconcelos Silva , Maeli Dal-Pai-Silva , Henrique Nunes de Oliveira , Luis Artur Loyola Chardulo
The objective of this study was to evaluate the association of expression of CAPN1, CAPN2, CAST, HSP90AA1, DNAJA1 and HSPB1 genes with meat tenderness in Nellore cattle. Three experimental groups were selected by shear force (SF): moderately tender (SF = 34.3 ± 5.8 N), moderately tough (SF = 56.8 ± 7.8 N), and very tough meat (SF = 80.4 ± 15 N). Gene expression was evaluated by real-time PCR. Expression of the CAPN1, CAPN2, CAST and CAST1 genes did not differ between groups. Expression of the CAST2 was up-regulated (P < 0.05) in the moderately tough and very tough meat groups. Down-regulation of the HSP90AA1, DNAJA1 and HSPB1 genes (P < 0.05) was observed in the moderately tender meat group. The present results suggest that meat tenderness in Nellore cattle does not directly depend on the expression of the CAPN1 and CAPN2 genes, but is associated with the expression of other genes such as CAST2, HSP90AA1, DNAJA1 and HSPB1.
中文翻译:
协会硬脂酸钙,HSP90AA1,DNAJA1和HSPB1基因在内洛尔牛肉嫩
这项研究的目的是评估CAPN1,CAPN2,CAST,HSP90AA1,DNAJA1和HSPB1基因的表达与内罗尔牛肉嫩度的关系。通过剪切力(SF)选择三个实验组:中度嫩嫩(SF = 34.3±5.8 N),中度坚韧(SF = 56.8±7.8 N)和非常坚硬的肉类(SF = 80.4±15 N)。通过实时PCR评估基因表达。在表达CAPN1,CAPN2,CAST和CAST1基因组间没有差异。CAST2的表达上调(P <0.05)在中等韧性和非常韧性的肉类中。下调的的HSP90AA1,DNAJA1和HSPB1基因(P 适度嫩的肉组中观察到<0.05)。目前的结果表明,内罗尔牛的肉嫩度不直接取决于CAPN1和CAPN2基因的表达,而是与其他基因如CAST2,HSP90AA1,DNAJA1和HSPB1的表达有关。