当前位置：
X-MOL 学术
›
J. Proteome Res.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Haloferax volcanii Proteome Response to Deletion of a Rhomboid Protease Gene
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-02-05 00:00:00 , DOI: 10.1021/acs.jproteome.7b00530 Mariana I. Costa 1 , Micaela Cerletti 1 , Roberto A. Paggi 1 , Christian Trötschel 2 , Rosana E. De Castro 1 , Ansgar Poetsch 2, 3 , María I. Giménez 1
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-02-05 00:00:00 , DOI: 10.1021/acs.jproteome.7b00530 Mariana I. Costa 1 , Micaela Cerletti 1 , Roberto A. Paggi 1 , Christian Trötschel 2 , Rosana E. De Castro 1 , Ansgar Poetsch 2, 3 , María I. Giménez 1
Affiliation
|
Rhomboids are conserved intramembrane serine proteases involved in cell signaling processes. Their role in prokaryotes is scarcely known and remains to be investigated in Archaea. We previously constructed a rhomboid homologue deletion mutant (ΔrhoII) in Haloferax volcanii, which showed reduced motility, increased novobiocin sensitivity, and an N- glycosylation defect. To address the impact of rhoII deletion on H. volcanii physiology, the proteomes of mutant and parental strains were compared by shotgun proteomics. A total of 1847 proteins were identified (45.8% of H. volcanii predicted proteome), from which 103 differed in amount. Additionally, the mutant strain evidenced 99 proteins with altered electrophoretic migration, which suggested differential post-translational processing/modification. Integral membrane proteins that evidenced variations in concentration, electrophoretic migration, or semitryptic cleavage in the mutant were considered as potential RhoII targets. These included a PrsW protease homologue (which was less stable in the mutant strain), a predicted halocyanin, and six integral membrane proteins potentially related to the mutant glycosylation (S-layer glycoprotein, Agl15) and cell adhesion/motility (flagellin1, HVO_1153, PilA1, and PibD) defects. This study investigated for the first time the impact of a rhomboid protease on the whole proteome of an organism.
中文翻译：
Haloferax volcanii蛋白质组响应菱形蛋白酶基因的删除
菱形是保守的膜内丝氨酸蛋白酶，参与细胞信号转导过程。它们在原核生物中的作用鲜为人知，尚需在古细菌中进行研究。我们先前在Haloferax volcanii中构建了菱形同源缺失突变体（ΔrhoII），该突变体显示出降低的运动性，增加的新霉素敏感性和N-糖基化缺陷。为了解决影响rhoII删除的H. volcanii生理，突变体和亲本菌株的蛋白质组被猎枪蛋白质组学比较。总共鉴定出1847种蛋白质（占火山菌的45.8％预测蛋白质组），其数量相差103。另外，该突变菌株证明了99种蛋白质具有改变的电泳迁移，这表明差异的翻译后加工/修饰。在突变体中浓度，电泳迁移或半胰蛋白酶裂解表现出差异的整合膜蛋白被认为是潜在的RhoII靶标。其中包括PrsW蛋白酶的同源物（在突变菌株中不太稳定），预测的卤素花青素和可能与突变糖基化（S层糖蛋白，Agl15）和细胞粘附/运动相关的六个完整膜蛋白（鞭毛蛋白1，HVO_1153， PilA1和PibD）缺陷。这项研究首次调查了菱形蛋白酶对生物体整个蛋白质组的影响。
更新日期：2018-02-06
中文翻译：
Haloferax volcanii蛋白质组响应菱形蛋白酶基因的删除
菱形是保守的膜内丝氨酸蛋白酶，参与细胞信号转导过程。它们在原核生物中的作用鲜为人知，尚需在古细菌中进行研究。我们先前在Haloferax volcanii中构建了菱形同源缺失突变体（ΔrhoII），该突变体显示出降低的运动性，增加的新霉素敏感性和N-糖基化缺陷。为了解决影响rhoII删除的H. volcanii生理，突变体和亲本菌株的蛋白质组被猎枪蛋白质组学比较。总共鉴定出1847种蛋白质（占火山菌的45.8％预测蛋白质组），其数量相差103。另外，该突变菌株证明了99种蛋白质具有改变的电泳迁移，这表明差异的翻译后加工/修饰。在突变体中浓度，电泳迁移或半胰蛋白酶裂解表现出差异的整合膜蛋白被认为是潜在的RhoII靶标。其中包括PrsW蛋白酶的同源物（在突变菌株中不太稳定），预测的卤素花青素和可能与突变糖基化（S层糖蛋白，Agl15）和细胞粘附/运动相关的六个完整膜蛋白（鞭毛蛋白1，HVO_1153， PilA1和PibD）缺陷。这项研究首次调查了菱形蛋白酶对生物体整个蛋白质组的影响。
京公网安备 11010802027423号