Objective

Various commercial products are available for guided tissue regeneration (GTR) therapy; however, they do not combine biosafety with the ability to control cell function. The purpose of this study was to evaluate the physicochemical and biological characteristics of the novel bilayer biodegradable poly(lactic-co-glycolic acid) (PLGA) membrane, and to assess whether the bilayer PLGA membrane could be used for periodontal tissue regeneration.

Methods

Bilayer biodegradable membrane was fabricated thorough a two-step freezing and lyophilization process using PLGA solution. The characteristics of bilayer membranes were evaluated with respect to surface morphology, stability, mechanical strength, and operability for clinical use. Cell proliferation and osteogenic differentiation were investigated on the each surface of bilayer membrane. Then, these membranes were implanted to the rat calvaria bone defect models and evaluated their capability for tissue regeneration.

Results

Biodegradable membranes composed of the solid and porous layer were successfully prepared and the surface morphologies analyzed. Physicochemical analyses revealed that the membranes possessed enough stability and mechanical properties for clinical use. It was also confirmed that the solid layer inhibited cell proliferation and subsequent connective tissue invasion, while the inner layer promoted proliferation and osteogenic differentiation, thus resulting in bone regeneration in vivo.

Significance

The layering technology used to fabricate the bilayer polymer membrane could be applied in the developing of other novel biomaterials. The present study demonstrates that the bilayer biodegradable polymer membranes facilitate tissue regeneration in vivo, and therefore represent a prospective biomaterial for GTR therapy.

中文翻译：

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层状PLGA膜用于牙周组织再生的开发

客观的

各种商业产品可用于引导组织再生（GTR）治疗；但是，它们不能将生物安全性与控制细胞功能的能力结合在一起。本研究的目的是评估的物理化学和新的可生物降解的双分子层的聚（乳酸-生物学特性的共-glycolic乙酸）（PLGA）膜，并评估该双层PLGA膜是否可用于牙周组织再生。

方法

使用PLGA溶液通过两步冷冻和冻干过程制造了双层可生物降解膜。评估了双层膜的特性，包括表面形态，稳定性，机械强度和临床可操作性。在双层膜的每个表面上研究细胞增殖和成骨分化。然后，将这些膜植入大鼠颅骨骨缺损模型并评估其组织再生能力。

结果

成功地制备了由固体和多孔层组成的可生物降解膜，并对表面形貌进行了分析。物理化学分析表明，该膜具有足够的稳定性和机械性能，可用于临床。还证实了固体层抑制细胞增殖和随后的结缔组织浸润，而内层促进增殖和成骨分化，从而导致体内骨再生。

意义

用于制造双层聚合物膜的分层技术可应用于其他新型生物材料的开发。本研究表明双层可生物降解的聚合物膜促进体内组织再生，因此代表了一种有望用于GTR治疗的生物材料。