Abstract Recent discoveries regarding the regulation of the biofilm life cycle by bacterial signaling systems have identified novel strategies for manipulation of biofilm development to control the biofouling of membrane-based water purification systems. Nitric oxide (NO) signaling has been shown to induce dispersal of a wide range of single- and multi-species biofilms. However, the impact of NO-mediated biofilm dispersal on the taxa composition of natural communities as well as the potential selection for non-responding community members have rarely been addressed. Here, we investigated the effect of diethylenetriamine (DETA) NONOate, an NO donor with a long half-life, on biofilm dispersal of a bacterial community responsible for membrane biofouling to address this question. The biofilm of a complex community from a fouled industrial reverse osmosis (RO) membrane was dispersed over 50% by 500 μM of DETA NONOate treatment in a continuous flow system. Once-daily treatment with DETA NONOate in a laboratory-scale RO system demonstrated its anti-biofouling effect by delaying the transmembrane pressure increase during constant-flux filtration. Characterization of the bacterial communities of dispersed cells and remaining biofilm cells using a 16S Illumina MiSeq metabarcoding approach demonstrated that biofilm dispersal by DETA NONOate had no selection bias in the community.

中文翻译：

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一氧化氮处理反渗透生物污垢控制及其对细菌群落的影响

摘要 最近关于细菌信号系统调节生物膜生命周期的发现已经确定了操纵生物膜发展以控制基于膜的水净化系统的生物污染的新策略。一氧化氮 (NO) 信号已被证明可诱导广泛的单物种和多物种生物膜的扩散。然而，NO 介导的生物膜扩散对自然群落的分类群组成以及无反应群落成员的潜在选择的影响很少得到解决。在这里，我们研究了二亚乙基三胺 (DETA) NONOate（一种半衰期长的 NO 供体）对负责膜生物污染的细菌群落的生物膜扩散的影响，以解决这个问题。来自污染的工业反渗透 (RO) 膜的复杂群落的生物膜在连续流动系统中通过 500 μM 的 DETA NONOate 处理分散超过 50%。在实验室规模的 RO 系统中每天用 DETA NONOate 处理一次，通过延迟恒流量过滤过程中跨膜压力的增加，证明了其抗生物污垢作用。使用 16S Illumina MiSeq 元条形码方法对分散细胞和剩余生物膜细胞的细菌群落进行表征表明，DETA NONOate 的生物膜分散在群落中没有选择偏差。在实验室规模的 RO 系统中每天用 DETA NONOate 处理一次，通过延迟恒流量过滤过程中跨膜压力的增加，证明了其抗生物污垢作用。使用 16S Illumina MiSeq 元条形码方法对分散细胞和剩余生物膜细胞的细菌群落进行表征表明，DETA NONOate 的生物膜分散在群落中没有选择偏差。在实验室规模的 RO 系统中每天用 DETA NONOate 处理一次，通过延迟恒流量过滤过程中跨膜压力的增加，证明了其抗生物污垢作用。使用 16S Illumina MiSeq 元条形码方法对分散细胞和剩余生物膜细胞的细菌群落进行表征表明，DETA NONOate 的生物膜分散在群落中没有选择偏差。