A new fluorescent probe based on coumarin conjugate has been synthesized which shows high quantum yield because of strong ICT effect. The absorption and fluorescence spectra of probe were measured in a variety of solvents and the Stokes shifts were found to be in linear relationship with the orientation polarizability of the solvents. The fluorescence intensity of probe decreased with a rise in water content which could be an effective fluorescence water sensor in organic solvents. Moreover, the probe presents a highly linear ratiometric fluorescence response to water content under the excitation of ultraviolet light. The detection results by the ratiometric fluorescence probe are in good agreement with that by the well-established standard Karl Fischer method. This ratiometric fluorescent probe provides another accessible tool for the quantitative determination of water content and may replace the Karl Fischer method for specific applications.

合成了一种基于香豆素共轭物的新型荧光探针，由于其强大的ICT效应，该探针显示出高的量子产率。在多种溶剂中测量了探针的吸收光谱和荧光光谱，发现斯托克斯位移与溶剂的取向极化率呈线性关系。探针的荧光强度随着水含量的增加而降低，这可能是有机溶剂中有效的荧光水传感器。此外，在紫外光激发下，探针对水的含量呈现出高度线性的比例荧光响应。比例荧光探针的检测结果与孔的检测结果非常吻合-建立了标准的卡尔·费休方法。这种比例式荧光探针为定量测定水含量提供了另一种简便易行的工具，可以代替卡尔费休法进行特定应用。