Herein, we describe the synthesis and application of cross-linked polystyrene-based dual-function nano- and microparticles containing both fluorescent tags and metals. Despite containing a single dye, these particles exhibit a characteristic dual-band fluorescence emission. Moreover, these particles can be combined with different metal ions to obtain hybrid metallofluorescent particles. We demonstrate that these particles are easily nanofected into living cells, allowing them to be used for effective fingerprinting in multimodal fluorescence-based and mass spectrometry-based flow cytometry experiments. Likewise, the in situ reductions of the metal ions enable other potential uses of the particles as heterogeneous catalysts.

中文翻译：

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用于多峰应用的金属荧光纳米粒子

在这里，我们描述了包含荧光标签和金属的交联聚苯乙烯基双功能纳米和微粒的合成和应用。尽管包含一种染料，但这些颗粒仍显示出特征性的双波段荧光发射。而且，这些颗粒可以与不同的金属离子结合以获得杂化的金属荧光颗粒。我们证明了这些粒子很容易被纳米感染到活细胞中，使它们可用于基于多峰荧光和基于质谱的流式细胞术实验中的有效指纹识别。同样，金属离子的原位还原还可以将颗粒用作非均相催化剂的其他潜在用途。