Difference in Binding of Long- and Medium-Chain Fatty Acids with Serum Albumin: The Role of Macromolecular Crowding Effect,Journal of Agricultural and Food Chemistry

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Difference in Binding of Long- and Medium-Chain Fatty Acids with Serum Albumin: The Role of Macromolecular Crowding Effect
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-01-30 00:00:00 , DOI: 10.1021/acs.jafc.7b03548
Tian-Tian Zhu ₁ , Yan Zhang ₁ , Xing-An Luo ₁ , Shen-Zhi Wang ₁ , Ming-Qiang Jia ₁ , Zhong-Xiu Chen ₁

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Fatty acids (FAs) are transported by serum albumin in plasma. Studies have been undertaken to address the binding of MCFAs or LCFAs to human plasma albumin (HPA) and bovine serum albumin (BSA) by characterizing the binding affinities. Previous research on FA binding to serum albumin was usually performed in dilute solutions that are not sufficiently concentrated for the interpretation of the significance of the results under normal physiological conditions. How macromolecular crowded media affect fatty acids and bovine serum albumin (BSA) binding remains unknown. In this article, we investigated the mechanism of FA-BSA binding in a polyethylene glycol crowding environment by using thermodynamic and spectroscopic methods. Molecular crowding increased the binding constant for saturated medium-chain fatty acids (MCFAs) but significantly decreased the binding constant for unsaturated long-chain FAs. The binding sites tended to increase in all the cases. Further investigation revealed that crowding media might loosen the structure of BSA, facilitating MCFA-BSA binding. This research is useful for understanding the transportation of FAs by BSA under physiological conditions and may also help to control digestion by the eventual incorporation of macromolecular crowding agents into food formulations.

中文翻译：

长链和中链脂肪酸与血清白蛋白结合的差异：大分子拥挤效应的作用

血浆中的血清白蛋白转运脂肪酸（FAs）。已经进行研究以通过表征结合亲和力来解决MCFA或LCFA与人血浆白蛋白（HPA）和牛血清白蛋白（BSA）的结合。先前关于FA与血清白蛋白结合的研究通常是在稀溶液中进行的，稀溶液的浓度不足以解释正常生理条件下结果的意义。大分子拥挤介质如何影响脂肪酸和牛血清白蛋白（BSA）的结合仍然未知。在本文中，我们使用热力学和光谱方法研究了聚乙二醇拥挤环境中FA-BSA的结合机理。分子拥挤增加了饱和中链脂肪酸（MCFAs）的结合常数，但显着降低了不饱和长链FAs的结合常数。在所有情况下，结合位点倾向于增加。进一步的调查表明，拥挤的培养基可能会使BSA的结构松散，从而促进MCFA-BSA的结合。这项研究对于了解BSA在生理条件下对FA的运输很有用，并且还可能通过将大分子拥挤剂最终掺入食品配方中来帮助控制消化。

更新日期：2018-01-30

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