Boronic acid and its derivatives have shown special capability to cis-diols groups such as glycoprotein and carbohydrate, which has been widely investigated and applied in practical disease diagnosis. Fiber-optic surface plasmon resonance (SPR) sensors with tiny size are very accurate and convenient tools for monitoring the surface interaction in real time. In this paper, phenylboronic acids (PBA) were formed on the sensing surface by coating the fiber surface with a self-assembled monolayer (SAM) with long and short alkyl groups. Compared with commercial available 4-mercaptophenyboronic acid, PBA with long carbon chain provided more flexible space in binding process and achieved much higher sensitivity to glycoprotein. Furthermore, PBA SAM showed much stronger response for Concanavalin A (ConA) than the traditional protein-protein interaction. The limit of detection for ConA was as low as 0.29 nM by L-PBA SAM modified fiber-optic SPR sensors. Finally, we tested different regeneration solutions to remove glycoprotein from the sensor surface and found that only acidic environment was not enough and the protein denaturant such as ethanol or urea was essential for glycoprotein dissociation.

硼酸及其衍生物显示出特殊的顺式能力-二醇类，例如糖蛋白和碳水化合物，已被广泛研究并应用于实际疾病诊断中。纤巧的光纤表面等离子体激元共振（SPR）传感器是用于实时监控表面相互作用的非常准确和便捷的工具。在本文中，通过在纤维表面涂上具有长和短烷基的自组装单分子层（SAM），在传感表面上形成苯基硼酸（PBA）。与市售的4-巯基苯硼酸相比，具有长碳链的PBA在结合过程中提供了更大的弹性空间，并且对糖蛋白的敏感性更高。此外，与传统的蛋白质-蛋白质相互作用相比，PBA SAM对伴刀豆球蛋白A（ConA）的反应强得多。ConA的检出限低至0。L-PBA SAM改良的光纤SPR传感器为29 nM。最后，我们测试了不同的再生溶液以从传感器表面去除糖蛋白，结果发现仅酸性环境是不够的，而蛋白质变性剂（例如乙醇或尿素）对于糖蛋白的解离至关重要。