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Internalization studies on zeolite nanoparticles using human cells†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2018-01-05 00:00:00 , DOI: 10.1039/c7tb02534c
Natália Vilaça 1, 2, 3, 4, 5 , Ricardo Totovao 6, 7, 8, 9 , Eko Adi Prasetyanto 6, 7, 8, 9, 10 , Vera Miranda-Gonçalves 5, 11, 12, 13 , Filipa Morais-Santos 3, 14, 15, 16, 17 , Rui Fernandes 5, 18, 19, 20 , Francisco Figueiredo 5, 18, 19, 20 , Manuel Bañobre-López 5, 21, 22, 23, 24 , António M. Fonseca 1, 2, 3, 4, 5 , Luisa De Cola 6, 7, 8, 9 , Fátima Baltazar 3, 14, 15, 16, 17 , Isabel C. Neves 1, 2, 3, 4, 5
Affiliation  

Zeolites are crystalline porous materials with a regular framework which have non-toxic effects on a variety of human cell lines and have been explored for cell imaging and drug delivery. Understanding the interaction between zeolite nanoparticles and cells is imperative for improving their potentialities, since the process of internalization of these particles is still poorly understood. In this study, the intracellular trafficking and internalization kinetics of zeolite L into breast cancer cells and normal epithelial mammary cells were analysed using scanning electron microscopy (SEM), confocal microscopy and transmission electron microscopy (TEM). We also studied the involvement of endocytic pathways using two pharmacological inhibitors, chlorpromazine and dynasore. Zeolite nanoparticles were taken up by both cell types and the cellular uptake was fast, and started immediately after 5 min of incubation. Interestingly, the uptake was dependent on the cell type since in breast cancer cells it was faster and more efficient, with a higher number of nanoparticles being internalized by cancer cells over time, compared to that in the epithelial mammary cells. TEM results showed that the internalized nanoparticles were mainly localized in the cell vacuoles. The data obtained upon using endocytic pharmacological inhibitors suggest that the zeolite L uptake is mediated by caveolin.

中文翻译:

使用人类细胞对沸石纳米粒子的内在化研究

沸石是具有规则构架的结晶多孔材料,对多种人类细胞系均无毒作用,并且已被研究用于细胞成像和药物递送。了解沸石纳米粒子与细胞之间的相互作用对于提高其潜力至关重要,因为对这些粒子的内在化过程仍知之甚少。在这项研究中,使用扫描电子显微镜(SEM),共聚焦显微镜和透射电子显微镜(TEM)分析了沸石L进入乳腺癌细胞和正常上皮乳腺细胞的细胞内运输和内在化动力学。我们还研究了使用两种药理抑制剂氯丙嗪和dynasore对内吞途径的参与。两种细胞类型都吸收了沸石纳米颗粒,并且细胞吸收很快,并且在孵育5分钟后立即开始。有趣的是,摄取量取决于细胞类型,因为与上皮乳腺细胞相比,在乳腺癌细胞中摄取更快,更有效,并且随着时间的推移,更多的纳米颗粒被癌细胞内在化。TEM结果表明,内在的纳米颗粒主要位于细胞液泡中。使用内吞药理学抑制剂获得的数据表明沸石L的摄取是由小孔蛋白介导的。与上皮乳腺细胞相比,随着时间的推移,癌细胞会内化更多数量的纳米颗粒。TEM结果表明,内在的纳米颗粒主要位于细胞液泡中。使用内吞药理学抑制剂获得的数据表明沸石L的摄取是由小孔蛋白介导的。与上皮乳腺细胞相比,随着时间的推移,癌细胞会内化更多数量的纳米颗粒。TEM结果表明,内在的纳米颗粒主要位于细胞液泡中。使用内吞药理学抑制剂获得的数据表明沸石L的摄取是由小孔蛋白介导的。
更新日期:2018-01-05
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