Assay of Glomalin Using a Quartz Crystal Microbalance Biosensor,Electroanalysis

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Assay of Glomalin Using a Quartz Crystal Microbalance Biosensor
Electroanalysis ( IF 2.7 ) Pub Date : 2018-01-05 , DOI: 10.1002/elan.201700772
Miroslav Pohanka ₁ , Vitezslav Vlcek ₂

Affiliation

Glomalin is a soil protein abundantly occurring in the soil. In the current time, knowledge about glomalin is limited and there are also missing simple test for the determination of glomalin in the environment. This work is devoted to construction of a biosensor which is expected to be a simple device for the determination of glomalin in extracts from soil samples. The biosensor was constructed using an antibody against glomalin and piezoelectric quartz crystal microbalance (QCM) sensor platform allowing label free assay. Electrodes of QCM were activated using cysteamine and glutaraldehyde and finally, an antibody against glomalin was immobilized. Glomalin was acquired from various soil samples by extraction in an autoclave and its content was determined by a standard spectrophotometric test. Time necessary to bind sufficient amount of glomalin was discovered for the biosensor and four hours incubation interval corresponded with maximal efficacy. Limit of detection for the biosensor based assay was found to be equal to 3.40 μg/g which is enough to cover all the tested soil samples containing glomalin in a concentration from 291 μg/g to 3.47 mg/g. The assay also fully correlated with the standard tests. In a conclusion, the piezoelectric biosensor seems to be a suitable platform for the determination of glomalin in samples of environment origin. The method represents an improvement of the current analytical platforms that are based on measurement of total protein content in soil extract.

中文翻译：

石英晶体微量天平生物传感器测定glomalin

Glomalin是一种在土壤中大量存在的土壤蛋白质。当前，关于gloomalin的知识是有限的，并且还缺少用于确定环境中的gloomalin的简单测试。这项工作致力于构建一种生物传感器，该传感器有望成为测定土壤样品提取物中的gloomalin的简单装置。生物传感器是使用抗gloomalin的抗体和压电石英晶体微量天平（QCM）传感器平台构建的，可进行无标记测定。用半胱胺和戊二醛激活QCM电极，最后固定抗glomalin的抗体。通过在高压釜中提取从各种土壤样品中获得了glomalin，并通过标准分光光度法测定了其含量。对于生物传感器，发现了结合足够量的gloomalin所需的时间，并且四个小时的孵育间隔对应于最大功效。发现基于生物传感器的测定的检出限等于3.40μg/ g，足以覆盖所有含有291-3.43 / mg浓度的gloomalin的被测土壤样品。该测定也与标准测试完全相关。总之，压电生物传感器似乎是确定环境起源样品中的gloomalin的合适平台。该方法代表了对当前分析平台的一种改进，该平台基于对土壤提取物中总蛋白质含量的测量。发现基于生物传感器的测定的检出限等于3.40μg/ g，足以覆盖所有含有291-3.43 / mg浓度的gloomalin的被测土壤样品。该测定也与标准测试完全相关。总而言之，压电生物传感器似乎是确定环境起源样品中的gloomalin的合适平台。该方法代表了对当前分析平台的一种改进，该平台基于对土壤提取物中总蛋白质含量的测量。发现基于生物传感器的测定的检出限等于3.40μg/ g，足以覆盖所有含有291-3.43 / mg浓度的gloomalin的被测土壤样品。该测定也与标准测试完全相关。总而言之，压电生物传感器似乎是确定环境起源样品中的gloomalin的合适平台。该方法代表了对当前分析平台的一种改进，该平台基于对土壤提取物中总蛋白质含量的测量。压电生物传感器似乎是确定环境起源样品中的gloomalin的合适平台。该方法代表了对当前分析平台的一种改进，该平台基于对土壤提取物中总蛋白质含量的测量。压电生物传感器似乎是确定环境起源样品中的gloomalin的合适平台。该方法代表了对当前分析平台的一种改进，该平台基于对土壤提取物中总蛋白质含量的测量。

更新日期：2018-01-05

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