Surface-Modified Wooden-Tip Electrospray Ionization Mass Spectrometry for Enhanced Detection of Analytes in Complex Samples,Analytical Chemistry

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Surface-Modified Wooden-Tip Electrospray Ionization Mass Spectrometry for Enhanced Detection of Analytes in Complex Samples
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-01-17 00:00:00 , DOI: 10.1021/acs.analchem.7b03675
Bin Hu _{1,

2,

3} , Pui-Kin So _{3,

4} , Yunyun Yang ₅ , Jiewei Deng _{1,

6} , Yi-Ching Choi _{1,

3} , Tiangang Luan ₆ , Zhong-Ping Yao _{1,

3,

7}

Affiliation

State Key Laboratory of Chirosciences, Food Safety and Technology Research Centre and Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region, China
Institute of Mass Spectrometer and Atmospheric Environment, Jinan University, Guangzhou 510632, China
State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation) and Shenzhen Key Laboratory of Food Biological Safety Control, Shenzhen Research Institute of The Hong Kong Polytechnic University, Shenzhen 518057, China
University Research Facility in Life Sciences, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region, China
Guangdong Engineering and Technology Research Center for Ambient Mass Spectrometry, Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, Guangdong Institute of Analysis (China National Analytical Center Guangzhou), 100 Xianlie Middle Road, Guangzhou 510070, China
State Key Laboratory of Biocontrol, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, School of Life Sciences, Sun Yat-Sen University, 135 Xingangxi Road, Guangzhou 510275, China
Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, (Yanbian University) Ministry of Education, Yanji, Jilin 133002, China

Replacement of capillary with solid substrates for sample loading and ionization has created many new possibilities for electrospray ionization mass spectrometry (ESI-MS). Surface modification is an attractive strategy to enhance the analytical capability of solid-substrate ESI-MS and allow understanding the relationship between surface activity of solid substrates and analytical properties. In this study, we performed surface modification of wooden tips with hydrophobic (−C₁₈), basic (−NH₂), and acidic (−SO₃H) functional groups and applied various sampling methods, i.e., extractive sampling and direct loading, to comprehensively investigate the analytical properties of solid-substrate ESI-MS. Our results showed that, for the direct loading method, analytes with weak interactions with solid-substrate surface could be readily sprayed out for detection. While for the extractive sampling method, analytes strongly retained on solid-substrate surface could be selectively enriched and detected, and a washing step after sample loading could effectively remove unbound components for reducing interference. Overall, the insights on the effects of surface–analyte interactions on the analytical features obtained in this study could aid the development of surface-modified strategies for enhancing the analytical capability of solid-substrate ESI-MS.

中文翻译：

表面修饰的木尖电喷雾电离质谱用于增强复杂样品中分析物的检测

用固体基质代替毛细管进行样品加载和电离，为电喷雾电离质谱（ESI-MS）创造了许多新的可能性。表面改性是提高固体基质ESI-MS的分析能力并了解固体基质表面活性与分析性能之间关系的一种有吸引力的策略。在这项研究中，我们用疏水性（-C ₁₈），碱性（-NH ₂）和酸性（-SO ₃）对木质尖端进行了表面改性。H）官能团并应用了多种采样方法，即萃取采样和直接加载，以全面研究固体基质ESI-MS的分析性质。我们的结果表明，对于直接上样方法，与固体基质表面相互作用较弱的分析物可以很容易地喷出进行检测。对于萃取采样方法，可以选择性地富集和检测强烈保留在固体基质表面上的分析物，样品加载后的洗涤步骤可以有效去除未结合的成分，从而减少干扰。总体而言，对表面-分析物相互作用对本研究中获得的分析特征的影响的见解可以帮助开发表面修饰策略，以增强固体基质ESI-MS的分析能力。

更新日期：2018-01-17

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