Porous scaffolds based on alginate-polymethacrylate hybrid hydrogels intended for bone and cartilage regeneration were prepared through controlled calcium ions diffusion from an agar mould. The double interconnected network of such materials combines into a single porous structure maintained by both noncovalent crosslinks (calcium ions for alginate) and covalent crosslinks (polymethacrylate crosslinked by the addition of mixtures of mono and bifunctional monomers). The alginate component ensures the appropriate micro-environment to mimic the extra-cellular matrix, whereas the polymethacrylate improves the mechanical performances of the hybrid hydrogels, helping to overcome the mechanical limitations of the alginate component. Morphological characterization and porosity analysis of the hybrid scaffolds were assessed by scanning electron microscopy and micro-computed tomography. Relative concentration and distribution of calcium ions were evaluated by atomic absorption and dispersive X-ray analysis, respectively. Uniaxial compressive mechanical tests were conducted to evaluate the compressive elastic modulus of the hybrid hydrogels that was correlated with their swelling ratio and crosslinking degree. As was envisaged a much higher modulus (about seven times) was obtained for the hybrid Alg/HE hydrogel than with alginate alone.

通过从琼脂模具中控制钙离子的扩散，制备了基于藻酸盐-聚甲基丙烯酸酯杂化水凝胶的多孔支架，旨在用于骨骼和软骨的再生。这样的材料的双互连网络结合成由非共价交联（藻酸钙离子）和共价交联（通过添加单和双官能单体的混合物交联的聚甲基丙烯酸酯）维持的单个多孔结构。藻酸盐组分确保适当的微环境以模仿细胞外基质，而聚甲基丙烯酸酯改善了杂化水凝胶的机械性能，有助于克服藻酸盐组分的机械限制。混合支架的形态表征和孔隙度分析通过扫描电子显微镜和计算机断层扫描进行评估。钙离子的相对浓度和分布分别通过原子吸收和色散X射线分析进行评估。进行单轴压缩力学测试以评估杂化水凝胶的压缩弹性模量，该弹性模量与其溶胀率和交联度有关。如所设想的，与单独的藻酸盐相比，杂化的Alg / HE水凝胶获得了更高的模量（大约七倍）。进行单轴压缩力学测试以评估杂化水凝胶的压缩弹性模量，该弹性模量与其溶胀率和交联度有关。如所设想的，与单独的藻酸盐相比，杂化的Alg / HE水凝胶获得了更高的模量（大约七倍）。进行单轴压缩力学测试以评估杂化水凝胶的压缩弹性模量，该弹性模量与其溶胀率和交联度有关。如所设想的，与单独的藻酸盐相比，杂化的Alg / HE水凝胶获得了更高的模量（大约七倍）。