A novel and low-cost micro-sensor with a diameter of 12 μm was developed based on Titanium dioxide-Polyaniline/Silk fibroin fiber (TiO₂-PANI/SFF). TiO₂-PANI composites were deposited on surfaces of SFFs by in-situ polymerization. The microfibers were characterized by Scanning electron microscopy (SEM), Energy dispersive spectrometer (EDS), Raman spectrometer, Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD), and the formation mechanism of them was illustrated in detail. With ammonia (NH₃) as model gas, the TiO₂-PANI/SFF micro-sensor showed good sensing performance with response value of 0.82 and response time of 10 s to NH₃ of 100 μg/L. When the micro-sensors were applied for pork freshness evaluation, the output response values showed good correlation with the total volatile basic nitrogen (TVB-N) levels in pork (R² = 0.990), and the discriminant results of the micro-sensors for pork freshness based on linear discriminant analysis (LDA) showed the prediction accuracy of calibration and prediction set was 90.73% and 86.38%, respectively, which indicated the great potential of TiO₂-PANI/SFF micro-sensor for pork freshness evaluation.

基于二氧化钛-聚苯胺/丝素蛋白纤维（TiO ₂ -PANI / SFF），开发了一种直径为12μm的新型低成本微传感器。通过原位聚合将TiO ₂ -PANI复合材料沉积在SFF的表面上。通过扫描电子显微镜（SEM），能量色散谱仪（EDS），拉曼光谱仪，傅立叶变换红外光谱（FTIR）和X射线衍射（XRD）对微纤维进行了表征，并详细说明了它们的形成机理。以氨气（NH ₃）为模型气体，TiO ₂ -PANI / SFF微传感器具有良好的传感性能，响应值为0.82，对NH _3的响应时间为10 s。的浓度为100μg/ L。当微传感器用于猪肉新鲜度评估时，输出响应值与猪肉中总挥发性碱性氮（TVB-N）水平具有良好的相关性（R ²  = 0.990），并且微传感器的判别结果为基于线性判别分析（LDA）的猪肉新鲜度显示，校准和预测集的预测准确度分别为90.73％和86.38％，这表明TiO ₂ -PANI / SFF微传感器在猪肉新鲜度评估中具有巨大的潜力。