Circulating tumor cells (CTCs), as the cellular origin of metastasis, are cancer cells that break away from a primary tumor and circulate in the peripheral blood. And they provide a wealth of information about tumor phenotype. Here, this work reported a novel ultrasensitive immunoassay protocol for the detection of CTCs by using [email protected] nanoflowers ([email protected]) and AuNPs/Acetylene black (AuNPs/AB) nanomaterial. In the established approach, AuNPs/AB nanomaterial was used as substrate material to increase the specific surface area and enhance the conductivity of the gold electrode. Protein G was used for oriented immobilization of capture antibody, which strongly improved the capture efficiency of MCF-7 cells. The innovatively synthesized [email protected] by our group with high specific surface area and good biocompatibility were not only as the carriers of signal antibodies (Ab₂) but also catalyzed the reduction of H₂O₂, which effectually amplified the current signal. A linear relationship between current signals and the concentrations of CTCs was obtained in the range from 20 to 1×10⁶ cells mL⁻¹ and the detection limit is as low as 3 cells mL⁻¹ on condition of acceptable stability and reproducibility. Furthermore, the as-proposed cytosensor showed excellent performance in the detection of CTCs in human blood samples. These results suggest that the proposed cytosensor will be a promising application for accurately quantitative detection of CTCs.

循环肿瘤细胞（CTC）是转移的细胞起源，是癌细胞脱离原发肿瘤并在外周血中循环。他们提供了大量有关肿瘤表型的信息。在这里，这项工作报告了一种新颖的超灵敏免疫分析方法，可通过使用[受电子邮件保护的]纳米花（[受电子邮件保护的]）和AuNPs /乙炔黑（AuNPs / AB）纳米材料检测CTC。在已建立的方法中，AuNPs / AB纳米材料被用作基底材料以增加比表面积并增强金电极的电导率。蛋白G用于捕获抗体的定向固定，从而极大地提高了MCF-7细胞的捕获效率。₂）还催化了H ₂ O ₂的还原，从而有效地放大了电流信号。在可接受的稳定性和再现性的条件下，在20至1×10 ⁶细胞mL ^-1的范围内获得了电流信号与CTC浓度之间的线性关系，并且检测限低至3细胞mL ^-1。此外，所提出的细胞传感器在检测人类血液样品中的四氯化碳方面表现出优异的性能。这些结果表明，所提出的细胞传感器将是准确定量检测CTC的有前途的应用。