The APSES transcription factors have been identified as key regulators of fungal development and other biological processes in fungi. In the present study, the function of Ganoderma lucidum GlSwi6, a homolog of Saccharomyces cerevisiae Swi6, was characterized. RNAi was used to examine the function of GlSwi6 in G. lucidum. Silencing GlSwi6 resulted in multiple developmental defects, including reduced fungal growth and increased hyphal branching, and the GlSwi6-silenced strains did not exhibit primordium or fruiting body formation. In addition, the H₂O₂ and ganoderic-acid (GA) levels of the GlSwi6-silenced strains decreased approximately 50% and 25%, respectively, compared with those of the WT strain. Furthermore, the addition of H₂O₂ led to the recovery of the GA levels of GlSwi6-silenced strains, implying that GlSwi6 might regulate GA biosynthesis by regulating the intracellular ROS levels. Taken together, these results indicate that GlSwi6 is involved in fungal growth, development and GA biosynthesis in G. lucidum.

APSES转录因子已被鉴定为真菌中真菌发育和其他生物过程的关键调节因子。在本研究中，表征了酿酒酵母Swi6的同源物灵芝GlSwi6的功能。RNAi用于检测GlSwi6在灵芝中的功能。使GlSwi6沉默会导致多种发育缺陷，包括真菌生长减少和菌丝分支增加，并且被GlSwi6沉默的菌株没有表现出原基或子实体的形成。此外，GlSwi6的H ₂ O ₂和灵芝酸（GA）含量与WT菌株相比，沉默的菌株分别降低了约50％和25％。此外，H ₂ O _2的添加导致GlSwi6沉默的菌株的GA水平恢复，这暗示GlSwi6可能通过调节细胞内ROS水平来调节GA生物合成。综上所述，这些结果表明GlSwi6参与了灵芝中的真菌生长，发育和GA生物合成。