A screening method was explored for simultaneous analysis of 43 cephalosporins residues and their metabolites from four generations in shrimp utilizing a novel fully untargeted data acquisition method. Four generations of cephalosporins residues and their metabolites are extracted with online extraction simultaneously. Ultrahigh-performance liquid chromatography coupled to quadrupole-orbitrap high resolution mass spectrometry was used for to determine cephalosporins and their metabolites in ten shrimp species. Precision in terms of relative standard deviation (RSD) was under 6.3% for all compounds, and the extraction recoveries ranged from 85% to 108%. The detection capabilities ranged from 0.011 to 0.223 mg kg⁻¹. The fully untargeted vDIA acquisition way improves both selectivity and sensitivity for the compounds at trace levels, which is beneficial for screening performance.

探索了一种筛选方法，可利用新型完全无目标数据采集方法同时分析虾中四代人的43种头孢菌素残基及其代谢物。在线提取可同时提取四代头孢菌素残基及其代谢产物。采用超高效液相色谱-四极杆-轨道阱高分辨率质谱法测定十种虾类中的头孢菌素及其代谢产物。所有化合物的相对标准偏差（RSD）精度均低于6.3％，萃取回收率介于85％至108％之间。检测能力范围为0.011至0.223 mg kg ^-1。完全无针对性的vDIA采集方式可提高痕量化合物的选择性和灵敏度，这对筛选性能很有帮助。