A method to introduce target analytes to a chromatograph from a single drop of whole blood was investigated for minimally invasive monitoring of anionic pharmaceuticals. In this work, salicylate and loxoprofen were examined as organic anions. A micro ion extractor (MIE) has been developed for extraction of inorganic trace anions from whole blood, but this device is not suitable for extraction of pharmaceuticals. In the present study, we improved and optimized the MIE device for organic anion extraction. Various supported liquid membranes were evaluated for use as the ion transfer membrane, with each membrane placed between a droplet sample (donor) and an acceptor solution. A supported liquid membrane of porous polypropylene impregnated with 1-butanol was selected. In addition, the methods for electric field creation and electrode contact were examined to improve the characteristics of the MIE device. The current and extraction time were also optimized. With the optimized method, salicylate and loxoprofen were successfully extracted from a single drop of whole blood. Changes in the concentrations of these pharmaceuticals in blood over time were monitored after administration. As only 25 μL of whole blood was required for analysis, repeat measurements could be conducted to monitor changes in the concentrations. This MIE will be useful for monitoring pharmaceutical concentrations in blood.

研究了从单滴全血中将目标分析物引入色谱仪的方法，以对阴离子药物进行微创监测。在这项工作中，水杨酸盐和洛索洛芬被视为有机阴离子。已经开发了一种微离子提取器（MIE），用于从全血中提取无机痕量阴离子，但是该设备不适用于提取药物。在本研究中，我们改进和优化了用于有机阴离子萃取的MIE设备。评价了各种支持的液体膜用作离子转移膜，并将每个膜放置在液滴样品（供体）和受体溶液之间。选择用1-丁醇浸渍的多孔聚丙烯的支撑液膜。此外，研究了电场产生和电极接触的方法，以改善MIE器件的特性。电流和提取时间也得到了优化。通过优化的方法，从单滴全血中成功提取了水杨酸酯和洛索洛芬。给药后监测这些药物在血液中的浓度随时间的变化。由于仅需要25μL全血进行分析，因此可以进行重复测量以监测浓度的变化。该MIE可用于监测血液中的药物浓度。给药后监测这些药物在血液中的浓度随时间的变化。由于仅需要25μL全血进行分析，因此可以进行重复测量以监测浓度的变化。该MIE可用于监测血液中的药物浓度。给药后监测这些药物在血液中的浓度随时间的变化。由于仅需要25μL全血进行分析，因此可以进行重复测量以监测浓度的变化。该MIE可用于监测血液中的药物浓度。