Characterization of Proteins Present in Isolated Senile Plaques from Alzheimer’s Diseased Brains by MALDI-TOF MS with MS/MS,ACS Chemical Neuroscience

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Characterization of Proteins Present in Isolated Senile Plaques from Alzheimer’s Diseased Brains by MALDI-TOF MS with MS/MS
ACS Chemical Neuroscience ( IF 4.1 ) Pub Date : 2018-01-04 00:00:00 , DOI: 10.1021/acschemneuro.7b00445
Andrea R. Kelley ₁ , George Perry ₂ , Stephan B. H. Bach ₁

Affiliation

The increase of insoluble senile plaques in the brain is a primary hallmark of Alzheimer’s disease. The usefulness of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with tandem MS for the characterization of senile plaques from AD brains and the relevance of the components identified to furthering AD research using MS is discussed. Thirty-three components were reproducibly observed within tryptic aliquots of senile plaques from two different AD brains after sample preparation optimization. Additionally, this is one of the first accounts of LIFT being utilized for the direct sequencing of peptides from isolated senile plaques. While many of the species observed coisolated within senile plaques have been linked to AD etiology, if only speculatively, this is the first instance that many of them have been demonstrated to be a part of the plaques themselves. This work is the first step in determining the potential roles that the species may have in the aggregation or proliferation of the plaques.

中文翻译：

MALDI-TOF MS-MS / MS表征阿尔茨海默氏病患大脑中分离出的老年斑中的蛋白质

大脑中不溶性老年斑的增加是阿尔茨海默氏病的主要特征。结合串联MS的基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）用于表征AD大脑中老年斑的有用性，并讨论了鉴定出的成分与进一步利用MS进行AD研究的相关性。优化样品制备后，在来自两个不同AD大脑的老年斑的胰蛋白酶等分试样中可重现地观察到33种成分。另外，这是LIFT最早用于从分离的老年斑中直接测序多肽的方法之一。虽然观察到的老年斑内共隔离的许多物种与AD病因有关，但仅是推测性的，这是首次证明其中许多是斑块本身的一部分。这项工作是确定物种在菌斑聚集或增殖中可能具有的潜在作用的第一步。

更新日期：2018-01-04

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