Water temperature is an important environmental factor that affects physiology and biochemical activities of fish. In this study, we investigated of high temperature on biochemical parameters, oxidative stress, DNA damage and apoptosis of pufferfish. Thermal stress could significantly increase the levels of AST, ALT, LDH, GLU and TG, whereas the levels of ALP and TP decrease significantly. In addition, thermal stress also decreased total blood cell count, inhibited cell viability, and subsequently lead to DNA damage and apoptosis. The mRNA levels of p53, caspase-9 and caspase-3 were up-regulated under thermal stress. These results suggested that caspase-dependent and p53 signaling pathways could play important roles in thermal stress-induced apoptosis in fish. Furthermore, the gene expression of SOD, CAT, HSP90 and C3 were induced by thermal stress. This study provides new insights into the mechanism whereby thermal stress affects physiological responses and apoptosis in pufferfish.

水温是影响鱼类生理和生化活动的重要环境因素。在这项研究中，我们调查了高温对河豚的生化参数，氧化应激，DNA损伤和细胞凋亡的影响。热应激可以显着增加AST，ALT，LDH，GLU和TG的水平，而ALP和TP的水平则显着降低。此外，热应激还减少了总血细胞计数，抑制了细胞活力，并随后导致DNA损伤和细胞凋亡。在热胁迫下，p53，caspase-9和caspase-3的mRNA水平上调。这些结果表明caspase依赖和p53信号通路可能在热应激诱导的鱼细胞凋亡中发挥重要作用。此外，SOD，CAT，HSP90和C3是由热应力引起的。这项研究为热应激影响河豚的生理反应和细胞凋亡提供了新的见解。