Cyanobacterial species produce wide range of bioactive compounds. This study characterized production of retinoid-like compounds with embryotoxic and teratogenic potential by commonly occurring cyanobacterial species with tendency to form massive water blooms. The major goal was to simultaneously assess the intracellular and extracellular retinoid-like activity from several independent cultivations of one coccal (Microcystis aeruginosa) and four filamentous cyanobacteria (Aphanizomenon gracile, Cylindrospermopsis raciborskii, Limnothrix redekeii, and Planktothrix agardhii) and characterize the variability in its production among cultivations. The retinoid-like activity was evaluated by in vitro assay along with chemical analyses of nine retinoids: all-trans retinoic acid (ATRA), 9-cis retinoic acid (9cis-RA), 13cis-RA, 13cis-RA methyl ester, 5,6 epoxy-RA, 4keto-ATRA, 4keto-retinal, 4hydoxy-retinoic acid (4OH-ATRA), retinal and retinol. The production of retinoid-like compounds was recalculated per volume, per biomass dry weight and per cell to provide relevant data for risk assessment in relation to occurrence of massive water blooms in the environment. Total produced retinoid-like activity of five selected species ranged from 170 to 25,600 ng ATRA-equivalents (REQ)/g dm corresponding to 0.001–0.392 ng REQ/10⁶ cyanobacterial cells. Results from chemical analyses showed that all tested extracts contained 4keto-ATRA and retinal. All-trans retinoic acid, 9/13cis-retinoic acid and 5,6 epoxy-retinoic acid were detected in most exudate and extract samples. The reported results of recalculated total retinoid-like activity enable potential predictions of its production by the studied species in water blooms of known cell densities relevant for risk assessment.

蓝细菌种类产生广泛​​的生物活性化合物。这项研究的特征是通过常见的蓝藻菌种具有形成大量水华的趋势，来生产类维生素A类化合物，具有胚胎毒性和致畸性。主要目标是同时评估几种独立培养的一种球菌（铜绿微囊藻）和四种丝状蓝细菌（Aphanizomenon gracile，Cylindrospermopsis raciborskii，Limnothrix redekeii和Planktothrix agardhii的特性）的细胞内和细胞外类维生素A活性，以及种植之间的生产。类维生素A活性通过体外评估测定和9种类维生素A的化学分析：全反式视黄酸（ATRA），9-顺式视黄酸（9cis-RA），13cis-RA，13cis-RA甲酯，5,6环氧-RA，4keto-ATRA， 4-酮-视网膜，4-羟基-视黄酸（4OH-ATRA），视网膜和视黄醇。类维生素A化合物的产量每体积，每生物质干重和每细胞重新计算一次，以提供与环境中大量水华发生有关的风险评估相关数据。五个选定物种的类视黄醇总活性为170至25,600 ng ATRA当量（REQ）/ g dm，对应于0.001–0.392 ng REQ / 10 ⁶蓝细菌细胞。化学分析的结果表明，所有测试的提取物均含有4keto-ATRA和视网膜。全部-在大多数渗出液和提取液样品中检测到反式视黄酸，9/13顺式视黄酸和5,6环氧-视黄酸。重新计算的类维生素A样总活性的报告结果使得研究物种在与风险评估相关的已知细胞密度的水华中潜在地预测了其产量。