A fast-growing fungus with remarkable ability to degrade several azo dyes under non-sterile conditions was isolated and identified. This fungus was identified as Trichoderma tomentosum. Textile effluent of ten-fold dilution could be decolorized by 94.9% within 72 h before optimization. Acid Red 3 R model wastewater with a concentration of 85.5 mg L⁻¹ could be decolorized by 99.2% within the same time after optimization. High-level of manganese peroxidase and low-level of lignin peroxidase activities were detected during the process of decolorization from the culture supernatant, indicating the possible involvement of two enzymes in azo dye decolorization. No aromatic amine products were detected from the degradation products of Acid Red 3 R by gas chromatography–mass spectrometry (GC/MS) analysis, indicating the possible involvement of a special symmetrical oxidative degradation pathway. Phytotoxicity assay confirmed the lower toxicity toward the test plant seeds of the degradation products when compared to the original dye.

分离并鉴定了一种快速生长的真菌，该真菌具有在非无菌条件下降解几种偶氮染料的显着能力。这种真菌被鉴定为毛木霉。在优化之前，经过十倍稀释的纺织品废水可以在72小时内脱色94.9％。浓度为85.5 mg L ^-1的酸性红3 R模型废水在优化后的同一时间内，可将其脱色99.2％。在从培养上清液脱色的过程中检测到高水平的锰过氧化物酶和低水平的木质素过氧化物酶活性，表明两种酶可能参与了偶氮染料的脱色。通过气相色谱-质谱法（GC / MS）分析，从酸性红3 R的降解产物中未检测到芳族胺产物，表明可能涉及特殊的对称氧化降解途径。植物毒性试验证实，与原始染料相比，降解产物对测试植物种子的毒性较低。