Monitoring the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG) during cell wall biosynthesis is correlated with bacterial taxonomy, physiology, micropathology, and antibacterial mechanisms. However, this is challenging because reported methods usually lack the ability of chiral analysis with the coexistence of d- and l-amino acids in PG. Here we report a chiral sensor array for PG biosynthesis monitoring through chiral amino acid recognition. Multitypes of host molecule modified MoS₂ nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing. By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously. The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.

中文翻译：

---

基于MoS ₂纳米片支持的主客体识别的手性传感器阵列，用于肽聚糖生物合成监测

监测细胞壁生物合成过程中细菌肽聚糖（PG）中手性和氨基酸种类的动态变化与细菌分类学，生理学，微病理学和抗菌机制相关。然而，这是有挑战性的，因为所报道的方法通常缺乏手性分析的能力以及PG中d-和l-氨基酸的共存。在这里，我们报告通过手性氨基酸识别的手性传感器阵列进行PG生物合成监测。多种类型的宿主分子修饰的MoS ₂纳米片（MNSs）被用作受体单位，以实现更准确，更特异性的传感。通过应用指示剂置换策略，获得了独特且可重现的荧光响应模式，用于线性判别分析（LDA），可同时准确区分非手性Gly，19 l-氨基酸和相应的19 d-对映体。传感器阵列还已经用于识别细菌种类并跟踪PG的氨基酸组成的细微变化，包括在不同生长状态和外源d-氨基酸刺激下的生物合成过程中的手性和种类。