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Characterization of Nanoscale Loaded Liposomes Produced by 2D Hydrodynamic Flow Focusing
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2018-01-23 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00572 Selya Amrani 1 , Maryam Tabrizian 1
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2018-01-23 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00572 Selya Amrani 1 , Maryam Tabrizian 1
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This paper presents the continuous flow formation by two-dimensional (2D) hydrodynamic flow focusing (HFF) of nanosized liposomes in microfluidic systems. The size distribution and concentration of the nanosized liposomes, as well as the polydispersity index (PDI) and zeta potential (ZP) of the liposomal dispersions, were investigated under various flow rate ratios (FRRs) and lipid formulations, by the selective incorporation of either positively charged DDAB (didodecyl-dimethylammonium bromide) or negatively charged DOPG (1,2 dioleoyl-sn-glycero-3- phosphoglycerol) lipids to the main bilayer DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) constituent. The challenges of encapsulating an FITC (fluorescein isothiocyanate)-labeled LC-TAT peptide (long chain of transactivator of transcription peptide), which plays a direct role in the HIV regulation and transcription, overcame and could be achieved via one-step nanoliposomes synthesis, in order to validate the potential of this device as an all-in-one nanoparticle synthesis and loading platform. Liposomes with sizes ranging between 60 to 800 nm were produced with low polydispersity and high particle throughput from alteration of the flow rate ratio and lipid concentration. We introduced the use of nanoparticle tracking analysis (NTA) to estimate for the first time the throughput of microfluidic synthesized liposomal NPs by measuring quantitatively the concentration of the synthesized particles at the outlet. These measurements showed that stable and unilamellar liposomes are generated at a maximum concentration of 1740 × 108 particles/mL in less than 2 min, with higher FRR enabling the most rapid generation of liposomes with similar diameter and significant lower polydispersity index than those obtained by other batch techniques.
中文翻译：
二维流体动力流聚焦产生纳米负载脂质体的表征
本文提出了通过二维（2D）纳米脂质体在微流体系统中的流体动力学流动聚焦（HFF）的连续流动形成。纳米脂质体的大小分布和浓度，以及脂质体分散体的多分散指数（PDI）和Zeta电位（ZP），通过选择性掺入两种流速比（FRR）和脂质制剂进行了研究带正电荷的DDAB（双十二烷基二甲基溴化铵）或带负电荷的DOPG（1,2油酰-sn-甘油-3-磷酸甘油）脂质带到主要双层DPPC（1,2-二棕榈酰-sn）-甘油-3-磷酸胆碱）成分。克服了FITC（异硫氰酸荧光素）标记的LC-TAT肽（转录肽的反式激活子的长链）的封装所面临的挑战，它在HIV调节和转录中起着直接作用，可以通过一步一步的纳米脂质体合成来克服，为了验证该设备作为多合一纳米颗粒合成和加载平台的潜力。通过改变流速比和脂质浓度，可制得尺寸范围在60至800 nm之间的脂质体，具有低多分散性和高颗粒通量。我们引入了使用纳米颗粒跟踪分析（NTA）来通过定量测量出口处合成颗粒的浓度来首次估算微流合成脂质体NP的通量的方法。在不到2分钟的时间内达到8颗粒/ mL，具有更高的FRR，与其他批次技术获得的脂质体相比，直径最大，多分散指数显着降低的脂质体可以最快速地生成。
更新日期：2018-01-23
中文翻译：
二维流体动力流聚焦产生纳米负载脂质体的表征
本文提出了通过二维（2D）纳米脂质体在微流体系统中的流体动力学流动聚焦（HFF）的连续流动形成。纳米脂质体的大小分布和浓度，以及脂质体分散体的多分散指数（PDI）和Zeta电位（ZP），通过选择性掺入两种流速比（FRR）和脂质制剂进行了研究带正电荷的DDAB（双十二烷基二甲基溴化铵）或带负电荷的DOPG（1,2油酰-sn-甘油-3-磷酸甘油）脂质带到主要双层DPPC（1,2-二棕榈酰-sn）-甘油-3-磷酸胆碱）成分。克服了FITC（异硫氰酸荧光素）标记的LC-TAT肽（转录肽的反式激活子的长链）的封装所面临的挑战，它在HIV调节和转录中起着直接作用，可以通过一步一步的纳米脂质体合成来克服，为了验证该设备作为多合一纳米颗粒合成和加载平台的潜力。通过改变流速比和脂质浓度，可制得尺寸范围在60至800 nm之间的脂质体，具有低多分散性和高颗粒通量。我们引入了使用纳米颗粒跟踪分析（NTA）来通过定量测量出口处合成颗粒的浓度来首次估算微流合成脂质体NP的通量的方法。在不到2分钟的时间内达到8颗粒/ mL，具有更高的FRR，与其他批次技术获得的脂质体相比，直径最大，多分散指数显着降低的脂质体可以最快速地生成。
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