Abstract The electrochemical immunosensor was fabricated by immobilizing alpha-fetoprotein (AFP) antigen onto the magnetic glassy carbon electrode (MGCE) that modified by Fe3O4@polystyrene (PS)/polydopamine (PDA)-Au nanotubes. The aim of this study was to develop a new route to prepare Fe3O4@PS/PDA-Au nanotubes with well dispersed Au nanoparticles (AuNPs). The immune detection platform showed good analytical performance for determination AFP in a linear range of 0.01–100 ng/mL with a correlation coefficient of 0.9970 under optimal conditions. The detection limit was calculated to 0.0088 ng/mL (S/N = 3). In addition, the good detection results of AFP in human serum were obtained by the immunosensor. The use of composite nanotubes to build advanced biosensing interface will bring more possibilities for future biomedical detection.

中文翻译：

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Fe 3 O 4 @PS/PDA-Au 纳米管的制备，用于甲胎蛋白的灵敏电化学检测

摘要 通过将甲胎蛋白（AFP）抗原固定在由Fe3O4@聚苯乙烯（PS）/聚多巴胺（PDA）-Au纳米管修饰的磁性玻碳电极（MGCE）上，制备了电化学免疫传感器。本研究的目的是开发一种制备具有良好分散的金纳米粒子 (AuNPs) 的 Fe3O4@PS/PDA-Au 纳米管的新途径。免疫检测平台显示出良好的分析性能，可在 0.01-100 ng/mL 的线性范围内测定 AFP，最佳条件下相关系数为 0.9970。检测限计算为 0.0088 ng/mL (S/N = 3)。此外，免疫传感器还获得了人血清中AFP的良好检测结果。利用复合纳米管构建先进的生物传感界面，将为未来的生物医学检测带来更多的可能性。