Developing a novel peroxidase nano-mimetic is a challenging research topic in biosensing field. Herein, WS₂ nanosheets (WS₂ NS) decorated with silver nanoclusters (AgNCs) was introduced as a new nanocomposite with improved peroxidase mimetic behavior. WS₂ NS/AgNCs nanocomposite was synthesized by simple chemical reduction of silver cations in the presence of WS₂ NS. The enhanced catalytic activity of nanocomposite in chemical and electrochemical reduction of H₂O₂ was studied using colorimetry, fluorometry and electrochemical techniques. Attaching the AgNCs on the surface of WS₂ NS effectively improved the catalytic activity of these nanosheets, which may be connected to the difference of the Fermi energy levels of coupled nanomaterial. The unequal Fermi levels cause charge separation between two phases creating highly active sites on the interface of coupled nanomaterial. Moreover, the new mimetic nanocomposite was applied for the analysis of glucose in blood, based on its enzymatic oxidation using glucose oxidase and then, on the measurement of produced H₂O₂ by sensitive fluorescence detection system. In optimum condition, a linear association was found between the generated fluorescence intensity and glucose logarithmic concentration in the range of 0.05–400 µM, and the limit of detection (3S/m) was 21 nM.

开发一种新型的过氧化物酶纳米模拟物是生物传感领域一个具有挑战性的研究课题。在这里，介绍了用银纳米团簇（AgNCs）装饰的WS ₂纳米片（WS ₂ NS）作为具有改进的过氧化物酶模拟行为的新型纳米复合材料。WS ₂ NS / AgNCs纳米复合材料是通过在WS ₂ NS存在下简单化学还原银阳离子而合成的。使用比色法，荧光法和电化学技术研究了纳米复合材料在化学和电化学还原H ₂ O ₂中增强的催化活性。将AgNC附着在WS ₂的表面上NS有效地改善了这些纳米片的催化活性，这可能与偶联纳米材料的费米能级的差异有关。费米能级不相等会导致两相之间的电荷分离，从而在耦合纳米材料的界面上产生高活性位点。此外，这种新型模拟纳米复合材料基于葡萄糖氧化酶的酶促氧化作用，然后通过灵敏的荧光检测系统测量产生的H ₂ O ₂，用于分析血液中的葡萄糖。在最佳条件下，发现荧光强度与葡萄糖对数浓度在0.05–400 µM之间呈线性关系，检出限（3S / m）为21 nM。