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Fluorescent Immunoassay for the Detection of Pathogenic Bacteria at the Single-Cell Level Using Carbon Dots-Encapsulated Breakable Organosilica Nanocapsule as Labels
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-01-16 00:00:00 , DOI: 10.1021/acsami.7b18714 Lu Yang 1 , Wenfang Deng 1 , Chang Cheng 1 , Yueming Tan 1 , Qingji Xie 1 , Shouzhuo Yao 1
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-01-16 00:00:00 , DOI: 10.1021/acsami.7b18714 Lu Yang 1 , Wenfang Deng 1 , Chang Cheng 1 , Yueming Tan 1 , Qingji Xie 1 , Shouzhuo Yao 1
Affiliation
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Herein, carbon dots (CDs)-encapsulated breakable organosilica nanocapsules (BONs) were facilely prepared and used as advanced fluorescent labels for ultrasensitive detection of Staphylococcus aureus. The CDs were entrapped in organosilica shells by cohydrolyzation of tetraethyl orthosilicate and bis[3-(triethoxysilyl)propyl]disulfide to form core–shell [email protected], where hundreds of CDs were encapsulated in each nanocapsule. Immunofluorescent nanocapsules, i.e., anti-S. aureus antibody-conjugated [email protected], were prepared to specifically recognize S. aureus. Before fluorescent detection, CDs were released from the BONs by simple NaBH4 reduction. The fluorescent signals were amplified by 2 orders of magnitude because of hundreds of CDs encapsulated in each nanocapsule, compared with a conventional immunoassay using CDs as fluorescent labels. A linear range was obtained at the S. aureus concentration from 1 to 200 CFU mL–1. [email protected] are also expected to expand to other systems and allow the detection of ultralow concentrations of targets.
中文翻译：
荧光免疫分析法在单细胞水平上检测病原细菌，使用碳点包封的易碎有机硅纳米胶囊作为标记
本文中，方便地制备了碳点（CDs）封装的易碎有机二氧化硅纳米胶囊（BONs），并用作高级荧光标记用于金黄色葡萄球菌的超灵敏检测。通过原硅酸四乙酯和双[3-（三乙氧基甲硅烷基）丙基]二硫化物的共水解，将CD截留在有机硅壳中，形成核-壳[电子邮件保护]，其中数百个CD封装在每个纳米胶囊中。制备了免疫荧光纳米胶囊，即抗金黄色葡萄球菌抗体偶联的[电子邮件保护的]，以特异性识别金黄色葡萄球菌。在进行荧光检测之前，CD通过简单的NaBH 4从BON中释放出来。减少。与将CD用作荧光标记的常规免疫分析相比，由于每个纳米胶囊中封装了数百个CD，荧光信号被放大了2个数量级。在金黄色葡萄球菌浓度为1至200 CFU mL –1时获得线性范围。[受电子邮件保护]还有望扩展到其他系统，并允许检测超低浓度的目标。
更新日期：2018-01-16
中文翻译：
荧光免疫分析法在单细胞水平上检测病原细菌，使用碳点包封的易碎有机硅纳米胶囊作为标记
本文中，方便地制备了碳点（CDs）封装的易碎有机二氧化硅纳米胶囊（BONs），并用作高级荧光标记用于金黄色葡萄球菌的超灵敏检测。通过原硅酸四乙酯和双[3-（三乙氧基甲硅烷基）丙基]二硫化物的共水解，将CD截留在有机硅壳中，形成核-壳[电子邮件保护]，其中数百个CD封装在每个纳米胶囊中。制备了免疫荧光纳米胶囊，即抗金黄色葡萄球菌抗体偶联的[电子邮件保护的]，以特异性识别金黄色葡萄球菌。在进行荧光检测之前，CD通过简单的NaBH 4从BON中释放出来。减少。与将CD用作荧光标记的常规免疫分析相比，由于每个纳米胶囊中封装了数百个CD，荧光信号被放大了2个数量级。在金黄色葡萄球菌浓度为1至200 CFU mL –1时获得线性范围。[受电子邮件保护]还有望扩展到其他系统，并允许检测超低浓度的目标。
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