A lysosome-targeting colorimetric and fluorescent dual signal probe for sensitive detection and bioimaging of hydrogen sulfide†,Analytical Methods

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A lysosome-targeting colorimetric and fluorescent dual signal probe for sensitive detection and bioimaging of hydrogen sulfide†
Analytical Methods ( IF 2.7 ) Pub Date : 2018-01-04 00:00:00 , DOI: 10.1039/c7ay02492d
Cuiyan Wu _{1,

2,

3,

4,

5} , Xiaojun Hu _{1,

2,

3,

4,

5} , Biao Gu _{1,

2,

3,

4,

5} , Peng Yin _{1,

2,

3,

4,

5} , Wei Su _{1,

2,

3,

4,

5} , Yaqian Li _{1,

2,

3,

4,

5} , Qiujun Lu _{1,

2,

3,

4,

5} , Youyu Zhang _{1,

2,

3,

4,

5} , Haitao Li _{1,

2,

3,

4,

5}

Affiliation

In this study, we developed a lysosome-targeting fluorescent probe, 3-(2,4-dinitrophenoxy)-2-(4-morpholinophenyl)-4H-chromen-4-one (DMC), for the detection of hydrogen sulfide (H₂S) based on excited-state intramolecular proton transfer (ESIPT). Upon the treatment with H₂S, a significant enhancement in the fluorescence was observed along with a distinct color change from yellow to colorless. This probe shows a low background spectroscopic signal, large Stokes shift, good sensitivity, and high selectivity towards H₂S. Most importantly, DMC has low toxicity and excellent cell permeability, and could be applied to visualizing H₂S in lysosomes.

中文翻译：

靶向溶酶体的比色和荧光双信号探针，用于硫化氢的灵敏检测和生物成像†

在这项研究中，我们开发了靶向溶酶体的荧光探针3-（2,4-二硝基苯氧基）-2-（4-吗啉代苯基）-4 H-铬-4--4-酮（DMC），用于检测硫化氢（ H ₂ S）基于激发态分子内质子转移（ESIPT）。用H ₂ S处理后，观察到荧光显着增强，并且颜色从黄色变为无色。该探针示出了低的背景光谱信号，大的斯托克斯频移，良好的灵敏度，和高选择性朝ħ ₂ S.最重要的是，DMC具有低毒性和优良的细胞渗透性，并且可以适用于可视化ħ ₂在溶酶体S上。

更新日期：2018-01-04

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