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Acoustically modulated biomechanical stimulation for human cartilage tissue engineering†‡
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-01-04 00:00:00 , DOI: 10.1039/c7lc01195d Umesh S. Jonnalagadda 1, 2, 3, 4, 5 , Martyn Hill 1, 2, 3, 4, 5 , Walid Messaoudi 1, 2, 3, 4, 5 , Richard B. Cook 1, 2, 3, 4, 5 , Richard O. C. Oreffo 3, 6, 7, 8, 9 , Peter Glynne-Jones 1, 2, 3, 4, 5 , Rahul S. Tare 1, 2, 3, 4, 5
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-01-04 00:00:00 , DOI: 10.1039/c7lc01195d Umesh S. Jonnalagadda 1, 2, 3, 4, 5 , Martyn Hill 1, 2, 3, 4, 5 , Walid Messaoudi 1, 2, 3, 4, 5 , Richard B. Cook 1, 2, 3, 4, 5 , Richard O. C. Oreffo 3, 6, 7, 8, 9 , Peter Glynne-Jones 1, 2, 3, 4, 5 , Rahul S. Tare 1, 2, 3, 4, 5
Affiliation
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Bioacoustofluidics can be used to trap and levitate cells within a fluid channel, thereby facilitating scaffold-free tissue engineering in a 3D environment. In the present study, we have designed and characterised an acoustofluidic bioreactor platform, which applies acoustic forces to mechanically stimulate aggregates of human articular chondrocytes in long-term levitated culture. By varying the acoustic parameters (amplitude, frequency sweep, and sweep repetition rate), cells were stimulated by oscillatory fluid shear stresses, which were dynamically modulated at different sweep repetition rates (1–50 Hz). Furthermore, in combination with appropriate biochemical cues, the acoustic stimulation was tuned to engineer human cartilage constructs with structural and mechanical properties comparable to those of native human cartilage, as assessed by immunohistology and nano-indentation, respectively. The findings of this study demonstrate the capability of acoustofluidics to provide a tuneable biomechanical force for the culture and development of hyaline-like human cartilage constructs in vitro.
中文翻译：
声调制生物力学刺激，用于人体软骨组织工程† ‡
生物声流体可用于捕获和悬浮流体通道中的细胞，从而有助于在3D环境中进行无支架的组织工程。在本研究中，我们设计并表征了一种声流生物反应器平台，该平台可在长期悬浮培养中应用声力机械刺激人类关节软骨细胞的聚集体。通过改变声学参数（幅度，扫频和扫频重复频率），细胞受到振荡的流体剪切应力的刺激，这些应力在不同的扫频重复频率（1–50 Hz）下被动态调制。此外，结合适当的生化提示，调整了声刺激，以工程改造具有与天然人软骨相当的结构和机械特性的人软骨构造，如分别通过免疫组织学和纳米压痕评估。这项研究的结果表明，声流流体能够为透明样人软骨构建体的培养和发展提供可调节的生物力学力。体外。
更新日期：2018-01-04
中文翻译：
声调制生物力学刺激，用于人体软骨组织工程† ‡
生物声流体可用于捕获和悬浮流体通道中的细胞，从而有助于在3D环境中进行无支架的组织工程。在本研究中，我们设计并表征了一种声流生物反应器平台，该平台可在长期悬浮培养中应用声力机械刺激人类关节软骨细胞的聚集体。通过改变声学参数（幅度，扫频和扫频重复频率），细胞受到振荡的流体剪切应力的刺激，这些应力在不同的扫频重复频率（1–50 Hz）下被动态调制。此外，结合适当的生化提示，调整了声刺激，以工程改造具有与天然人软骨相当的结构和机械特性的人软骨构造，如分别通过免疫组织学和纳米压痕评估。这项研究的结果表明，声流流体能够为透明样人软骨构建体的培养和发展提供可调节的生物力学力。体外。
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