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Simultaneous saccharification and aerobic fermentation of high titer cellulosic citric acid by filamentous fungus Aspergillus niger
Bioresource Technology ( IF 9.7 ) Pub Date : 2018-01-04 , DOI: 10.1016/j.biortech.2018.01.011
Weiliang Hou , Jie Bao

Simultaneous saccharification and fermentation (SSF) is the most efficient operation in biorefining conversion, but aerobic SSF under high solids loading significantly faces the serious oxygen transfer limitation. This study took the first insight into an aerobic SSF by high oxygen demanding filamentous fungi in highly viscous lignocellulose hydrolysate. The results show that oxygen requirement in the aerobic SSF by was well satisfied for production of cellulosic citric acid. The record high citric acid titer of 136.3 g/L and the overall conversion yield of 74.9% of cellulose were obtained by the aerobic SSF. The advantage of SSF to the separate hydrolysis and fermentation (SHF) on citric acid fermentation was compared based on the rigorous Aspen Plus modeling. The techno-economic analysis indicates that the minimum citric acid selling price (MCSP) of $0.603 per kilogram by SSF was highly competitive with the commercial citric acid from starch feedstock.

中文翻译:


丝状真菌黑曲霉同时糖化好氧发酵高滴度纤维素柠檬酸



同步糖化发酵(SSF)是生物精炼转化中最有效的操作,但高固体负荷下的好氧SSF显着面临严重的氧传递限制。这项研究首次深入了解了高粘性木质纤维素水解产物中高需氧丝状真菌的需氧 SSF。结果表明,好氧SSF中的氧气需求很好地满足了纤维素柠檬酸的生产。好氧SSF获得了创纪录的136.3 g/L柠檬酸滴度和74.9%的纤维素总转化率。基于严格的 Aspen Plus 建模,比较了 SSF 与柠檬酸发酵分离水解发酵 (SHF) 的优势。技术经济分析表明,SSF 的柠檬酸最低销售价格 (MCSP) 为每公斤 0.603 美元,与来自淀粉原料的商业柠檬酸相比具有很强的竞争力。
更新日期:2018-01-04
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