Protein Handshake on the Nanoscale: How Albumin and Hemoglobin Self-Assemble into Nanohybrid Fibers,ACS Nano

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Protein Handshake on the Nanoscale: How Albumin and Hemoglobin Self-Assemble into Nanohybrid Fibers
ACS Nano ( IF 15.8 ) Pub Date : 2018-01-12 00:00:00 , DOI: 10.1021/acsnano.7b07196
Christian Helbing ₁ , Tanja Deckert-Gaudig ₂ , Izabela Firkowska-Boden ₁ , Gang Wei ₃ , Volker Deckert _{2,

4} , Klaus D. Jandt _{1,

5,

6}

Affiliation

Creating and establishing proof of hybrid protein nanofibers (hPNFs), i.e., PNFs that contain more than one protein, is a currently unsolved challenge in bioinspired materials science. Such hPNFs could serve as universal building blocks for the bottom-up preparation of functional materials with bespoke properties. Here, inspired by the protein assemblies occurring in nature, we introduce hPNFs created via a facile self-assembly route and composed of human serum albumin (HSA) and human hemoglobin (HGB) proteins. Our circular dichroism results shed light on the mechanism of the proteins’ self-assembly into hybrid nanofibers, which is driven by electrostatic/hydrophobic interactions between similar amino acid sequences (protein handshake) exposed to ethanol-triggered protein denaturation. Based on nanoscale characterization with tip-enhanced Raman spectroscopy (TERS) and immunogold labeling, our results demonstrate the existence and heterogenic nature of the hPNFs and reveal the high HSA/HGB composition ratio, which is attributed to the fast self-assembling kinetics of HSA. The self-assembled hPNFs with a high aspect ratio of over 100 can potentially serve as biocompatible units to create larger bioactive structures, devices, and sensors.

中文翻译：

纳米尺度上的蛋白质握手：白蛋白和血红蛋白如何自组装成纳米混杂纤维

创建和建立混合蛋白纳米纤维（hPNFs）的证明，我。例如，含有一种以上蛋白质的PNF在生物启发性材料科学中是目前尚未解决的挑战。此类hPNF可以用作通用的构建块，用于自底向上制备具有定制属性的功能材料。在这里，受自然界中蛋白质组装的启发，我们介绍了通过一种简便的自组装路线，由人血清白蛋白（HSA）和人血红蛋白（HGB）蛋白质组成。我们的圆二色性结果揭示了蛋白质自组装为杂合纳米纤维的机制，这是由暴露于乙醇触发的蛋白质变性的相似氨基酸序列（蛋白质握手）之间的静电/疏水相互作用驱动的。基于尖端增强拉曼光谱（TERS）和免疫金标记的纳米级表征，我们的结果证明了hPNF的存在和异质性，并揭示了高HSA / HGB组成比，这归因于HSA的快速自组装动力学。具有超过100的高长宽比的自组装hPNF可以潜在地用作生物相容性单元，以创建更大的生物活性结构，设备和传感器。

更新日期：2018-01-12

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