Abstract Waste paper has a potential to serve as renewable feedstock for the biorefineries of fuels, chemicals and materials due to rich in cellulose and its abundance at low cost. In the present study, pretreated waste office paper (WOP) was enzymatically hydrolysed and used for lipid production by Cryptococcus curvatus . The results suggested that the WOP hydrolysate supplemented with ammonium sulphate (2 g/L) and yeast extract (0.5 g/L) as nitrogen source at a C/N ratio of 80 were the most suitable for high yield of lipids. The biomass, lipid yield, lipid content and lipid coefficient achieved from batch cultivation of C. curvatus using untreated and pretreated WOP hydrolysates were 6.32 and 15.20 g/L, 1.39 and 5.75 g/L, 22 and 37.8%, and 99.9 and 234.6 mg/g sugar with the productivity of 0.02 and 0.08 g/L/h, respectively. The fatty acid profile of the lipids indicated that the oleic acid was the major fatty acid followed by palmitic acid, stearic acid and linoleic acid which is quite similar to plant/vegetable oils. Thus, the results suggested that the waste office paper could be an alternative feedstock for production of microbial lipids for biodiesel.

中文翻译：

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产油酵母 Cryptococcus curvatus 从废办公纸中提高微生物脂质的生产

摘要 废纸具有作为燃料、化学品和材料生物精炼的可再生原料的潜力，因为它富含纤维素，而且成本低廉。在本研究中，经过预处理的办公废纸 (WOP) 被酶水解并用于弯曲隐球菌的脂质生产。结果表明，添加硫酸铵 (2 g/L) 和酵母提取物 (0.5 g/L) 作为氮源且 C/N 比为 80 的 WOP 水解液最适合高产脂质。使用未处理和预处理的 WOP 水解物分批培养弯管藻的生物量、脂质产量、脂质含量和脂质系数分别为 6.32 和 15.20 g/L、1.39 和 5.75 g/L、22% 和 37.8% 以及 99.9 和 234.6 mg /g 糖的生产率分别为 0.02 和 0.08 g/L/h。脂质的脂肪酸谱表明，油酸是主要的脂肪酸，其次是棕榈酸、硬脂酸和亚油酸，这与植物油/植物油非常相似。因此，结果表明废办公纸可以作为生产生物柴油微生物脂质的替代原料。