Functionalization and Characterization of Magnetic Nanoparticles for the Detection of Ferritin Accumulation in Alzheimer’s Disease,ACS Chemical Neuroscience

当前位置： X-MOL 学术 › ACS Chem. Neurosci. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Functionalization and Characterization of Magnetic Nanoparticles for the Detection of Ferritin Accumulation in Alzheimer’s Disease
ACS Chemical Neuroscience ( IF 4.1 ) Pub Date : 2018-01-03 00:00:00 , DOI: 10.1021/acschemneuro.7b00260
Tamara Fernández ₁ , Alberto Martínez-Serrano ₂ , Lorena Cussó _{3,

4,

5} , Manuel Desco _{3,

4,

5} , Milagros Ramos-Gómez _{1,

6}

Affiliation

Early diagnosis in Alzheimer’s disease (AD), prior to the appearance of marked clinical symptoms, is critical to prevent irreversible neuronal damage and neural malfunction that lead to dementia and death. Therefore, there is an urgent need to generate new contrast agents which reveal by a noninvasive method the presence of some of the pathological signs of AD. In the present study, we demonstrate for the first time a new nanoconjugate composed of magnetic nanoparticles bound to an antiferritin antibody, which has been developed based on the existence of iron deposits and high levels of the ferritin protein present in areas with a high accumulation of amyloid plaques (particularly the subiculum in the hippocampal area) in the brain of a transgenic mouse model with five familial AD mutations. Both in vitro and after intravenous injection, functionalized magnetic nanoparticles were able to recognize and bind specifically to the ferritin protein accumulated in the subiculum area of the AD transgenic mice.

中文翻译：

磁性纳米粒子的功能化和表征，用于检测阿尔茨海默氏病中铁蛋白的积累

在出现明显的临床症状之前，阿尔茨海默氏病（AD）的早期诊断对于防止不可逆的神经元损伤和导致痴呆症和死亡的神经功能衰竭至关重要。因此，迫切需要产生新的造影剂，其通过非侵入性方法揭示AD的一些病理征象的存在。在本研究中，我们首次证明了一种新的由磁性纳米颗粒与抗铁蛋白抗体结合的纳米共轭物，该结合物是根据铁沉积物的存在和高铁蛋白堆积区域中高水平的铁蛋白蛋白的存在而开发的。带有五个家族性AD突变的转基因小鼠模型的大脑中的淀粉样蛋白斑块（特别是海马区的下丘脑）。在体外和静脉注射后，

更新日期：2018-01-03

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11